Gao Feng, Li Yan, Liu Wei, Lu Xiang-lan, Li Xia, Wang Ping-ping, Liu Yun-peng
Department of Hematology, The First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Zhonghua Xue Ye Xue Za Zhi. 2006 Jan;27(1):25-7.
To study the relationship between E-cadherin gene expression and the methylation status of E-cadherin 5' CpG islands in acute myeloid leukemia (AML).
Reverse transcription-PCR (RT-PCR), flow cytometry and methylation specific PCR were used to analyze the E-cadherin gene and protein expression and its 5' CpG island methylation status respectively in bone marrow cells from 55 AML patients and 7 normal controls.
AML cells displayed a significant reduction or lack of E-cadherin gene and protein expression, the positive rates were 23.6% and 18.2% (P < 0.01), respectively. All normal bone marrow cells were E-cadherin positive. Thirty-eight of the 55 patients (69.1%) were E-cadherin 5' CpG island methylated whereas the normal controls were completely unmethylated. Twenty-nine of thirty-one (93.5%) E-cadherin-negative samples showed abnormal hypermethylation of the E-cadherin CpG islands.
Expression downregulation and methylation of E-cadherin gene in AML suggest that it might be an important event in AML. E-cadherin methylation was associated with the inhibition of E-cadherin gene and protein expression in AML.
研究急性髓系白血病（AML）中E-钙黏蛋白基因表达与E-钙黏蛋白5' CpG岛甲基化状态之间的关系。
采用逆转录聚合酶链反应（RT-PCR）、流式细胞术和甲基化特异性PCR分别分析55例AML患者和7例正常对照骨髓细胞中E-钙黏蛋白基因和蛋白表达及其5' CpG岛甲基化状态。
AML细胞中E-钙黏蛋白基因和蛋白表达显著降低或缺失，阳性率分别为23.6%和18.2%（P < 0.01）。所有正常骨髓细胞E-钙黏蛋白均呈阳性。55例患者中有38例（69.1%）E-钙黏蛋白5' CpG岛甲基化，而正常对照均未甲基化。31例E-钙黏蛋白阴性样本中有29例（93.5%）显示E-钙黏蛋白CpG岛异常高甲基化。
AML中E-钙黏蛋白基因表达下调和甲基化表明其可能是AML中的一个重要事件。E-钙黏蛋白甲基化与AML中E-钙黏蛋白基因和蛋白表达的抑制相关。
