Berger Ingrid, Stahl Sonja, Rychkova Natalia, Felbor Ute
Department of Human Genetics, University of Würzburg, Germany.
J Negat Results Biomed. 2006 Jun 1;5:8. doi: 10.1186/1477-5751-5-8.
Vascular endothelial growth factor (VEGF) and endostatin are angiogenic and anti-angiogenic molecules, respectively, that have been implicated in neurogenesis and neuronal survival. Using alkaline phosphatase fusion proteins, we show that the PC12 neuronal cell line contains cell membrane receptors for VEGF but not for endostatin and the collagen XV endostatin homologue. Immunocytochemistry confirmed that proliferating and differentiated PC12 cells express VEGF receptors 1, 2 and neuropilin-1. While no functional effects of VEGF on PC12 cell proliferation and differentiation could be observed, a slight VEGF-induced reduction of caspase-3 activity in differentiated apoptotic PC12 cells was paralleled by transient activation of ERK1/2 and Akt. In direct comparison, nerve growth factor proved to be a strikingly more potent neuroprotective agent than VEGF.
血管内皮生长因子（VEGF）和内皮抑素分别是促血管生成和抗血管生成分子，它们与神经发生和神经元存活有关。利用碱性磷酸酶融合蛋白，我们发现PC12神经元细胞系含有VEGF的细胞膜受体，但不含有内皮抑素和XV型胶原内皮抑素同源物的受体。免疫细胞化学证实，增殖和分化的PC12细胞表达VEGF受体1、2和神经纤毛蛋白-1。虽然未观察到VEGF对PC12细胞增殖和分化有功能影响，但在分化的凋亡PC12细胞中，VEGF诱导的半胱天冬酶-3活性略有降低，同时ERK1/2和Akt被短暂激活。直接比较表明，神经生长因子被证明是一种比VEGF更有效的神经保护剂。
