Zhang Ping-an, Wu Jian-min, Li Yan
Department of Laboratory Science, Renmin Hospital of Wuhan University, Wuhan 430060, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2006 Jan;27(1):41-3.
To explore the possible relationship between the polymorphisms of interferon-gamma (IFN-gamma) gene intron 1 at position +874 and the susceptibility of hepatitis B virus infection.
IFN-gamma gene single nucleotide polymorphism (intron 1 at position +874) were detected in 531 subjects including 231 chronic carrier of hepatitis B virus (group CC), 165 individuals who spontaneously recovered from HBV infection (group SR) and 135 healthy controls, by polymerase chain reaction with sequence specific primers.
Significant difference of IFN-gamma +874 AA genotype frequency was found between group CC (TT, TA and AA frequencies were 9.1%, 12.1% and 78.8%, respectively) and control group (TT, TA and AA frequencies were 12.6%, 23.0% and 64.4%, respectively) (chi(2) = 9.60, P = 0.008), but no significant difference found between group SR (TT, TA and AA frequencies were 13.9%, 23.7% and 62.4%, respectively) and control group (chi(2) = 0.16, P = 0.92).
There was association between IFN-gamma gene polymorphism (intron 1 at position +874) and chronic HBV infection. This study suggested the possibility that IFN-gamma gene polymorphism (intron 1 at position +874) might be important in determining an individual's susceptibility in the development of chronic HBV infection.
探讨干扰素-γ（IFN-γ）基因内含子1第+874位多态性与乙型肝炎病毒感染易感性之间的可能关系。
采用序列特异性引物聚合酶链反应，对531名受试者进行IFN-γ基因单核苷酸多态性（内含子1第+874位）检测，其中包括231例慢性乙型肝炎病毒携带者（CC组）、165例从HBV感染中自发康复的个体（SR组）和135名健康对照者。
CC组（TT、TA和AA基因型频率分别为9.1%、12.1%和78.8%）与对照组（TT、TA和AA基因型频率分别为12.6%、23.0%和64.4%）之间，IFN-γ +874 AA基因型频率存在显著差异（χ² = 9.60，P = 0.008），但SR组（TT、TA和AA基因型频率分别为13.9%、23.7%和62.4%）与对照组之间无显著差异（χ² = 0.16，P = 0.92）。
IFN-γ基因多态性（内含子1第+874位）与慢性HBV感染之间存在关联。本研究提示，IFN-γ基因多态性（内含子1第+874位）在决定个体发生慢性HBV感染的易感性方面可能具有重要作用。
