DU145人前列腺癌细胞系携带p53的一个温度敏感等位基因。
The DU145 human prostate carcinoma cell line harbors a temperature-sensitive allele of p53.
作者信息
Bajgelman Marcio C, Strauss Bryan E
机构信息
Viral Vector Group, Laboratory of Genetics and Molecular Cardiology, InCor, University of São Paulo School of Medicine, Brazil.
出版信息
Prostate. 2006 Sep 15;66(13):1455-62. doi: 10.1002/pros.20462.
BACKGROUND
Some nuances of mutant and wild-type p53 activity have been uncovered utilizing temperature sensitive (TS) alleles. However, few human tumor derived cell lines possess a TS p53 mutant.
METHODS
The cell lines DU145 (heterozygous p53, P223L, and V274F) and PC3 (p53-null, where exogenous P223L and V274F were introduced individually or in combination) were examined for TS p53 activity as revealed by reporter construct and target gene activation.
RESULTS
TS p53 function was observed in DU145 and expression of the P223L allele in PC3 conferred a TS p53 profile. Activation of p21Waf1 demonstrated that P223L TS activity may have been influenced by cellular context.
CONCLUSIONS
The DU145 cell line harbors a TS mutant of p53 and, in addition to being a widely used model of human prostate carcinoma, may also reveal new insights into p53 function due to the unique transcriptional properties of its TS phenotype.
背景
利用温度敏感（TS）等位基因已揭示了突变型和野生型p53活性的一些细微差别。然而，很少有源自人类肿瘤的细胞系具有TS p53突变体。
方法
检测了DU145细胞系（p53杂合，P223L和V274F）和PC3细胞系（p53缺失，单独或联合导入外源性P223L和V274F）的TS p53活性，通过报告基因构建体和靶基因激活来揭示。
结果
在DU145中观察到TS p53功能，并且PC3中P223L等位基因的表达赋予了TS p53特征。p21Waf1的激活表明P223L TS活性可能受细胞环境影响。
结论
DU145细胞系含有p53的TS突变体，并且除了作为广泛使用的人类前列腺癌模型外，由于其TS表型的独特转录特性，还可能揭示p53功能的新见解。
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