Siggins G R, Henriksen S J
Science. 1975 Aug 15;189(4202):559-61. doi: 10.1126/science.167439.
Cyclic adenosine monophosphate (cyclic AMP) and 11 derivatives were applied to rat cerebellar Purkinje cells by iontophoresis. Cyclic AMP inhibited 63 percent of the cells, while the 8-parachlorophenylthio- and 8-benzylthio- analogs of cyclin AMP inhibited the spontaneous firing of 92 and 89 percent of cells, respectively. The ability of the 11 analogs to inhibit neuronal firing correlated ( r= + .78) with their reported potency in activating cyclic AMP-dependent protein kinase. These results extend previous studies, pointing to the mediation by cyclic AMP of the noradrenergic inhibition of Purkinje neurons, and provide new physiological evidence that protein phosphorylation is a major step in the action of cyclic AMP.
通过离子电泳法将环磷酸腺苷（环AMP）及其11种衍生物施加于大鼠小脑浦肯野细胞。环AMP抑制了63%的细胞，而环AMP的8-对氯苯硫基和8-苄硫基类似物分别抑制了92%和89%细胞的自发放电。这11种类似物抑制神经元放电的能力与其在激活环AMP依赖性蛋白激酶方面的报告效力相关（r = +0.78）。这些结果扩展了先前的研究，表明去甲肾上腺素能对浦肯野神经元的抑制作用是由环AMP介导的，并提供了新的生理学证据，证明蛋白质磷酸化是环AMP作用的一个主要步骤。
