饲喂高蛋白和低蛋白饮食的小鼠肝脏中天冬氨酸转氨酶（AspAT）、α1抗胰蛋白酶（A1AT）、磷酰二肽酶A（FDPA）、苹果酸脱氢酶（MDH）、异柠檬酸脱氢酶（ICDH）和乳酸脱氢酶（LDH）活性的昼夜变化。

Diurnal changes in activities of AspAT, A1AT, FDPA, MDH, ICDH and LDH in liver of mice fed high and low protein diet.

Zaleska-Freljan K, Priebe T S

Department of Vertebrate Animal Physiology, University of Warsaw, Poland.

Comp Biochem Physiol A Comp Physiol. 1991;98(3-4):575-9. doi: 10.1016/0300-9629(91)90449-m.

Abstract

Diurnal changes of the activities of AspAT, A1AT, FDPA, MDH, ICDH and LDH in liver of laboratory mice were studied. 2. Experimental mice were fed granulated maize diet with a low (9%) protein content whereas control animals received the standard Murigran granulated diet with 25% protein content. 3. The experiment was held for 7 weeks and animals were kept in L:D = 12:12 at 24 +/- 2 degrees C conditions. 4. The liver samples were taken every 4 hours starting at 08.00 a.m. 5. Diurnal rhythm in activity of AspAT, A1AT, MDH and LDH was observed in both groups while diurnal rhythm in activity of FDPA was found only in experimental animals. 6. Diurnal rhythm in activity of ICDH was observed only in control mice. 7. The low protein maize diet did not change the rhythmicity of enzymes studied, except the FDPA and ICDH activities.

摘要

研究了实验小鼠肝脏中天冬氨酸氨基转移酶（AspAT）、α1抗胰蛋白酶（A1AT）、果糖二磷酸醛缩酶（FDPA）、苹果酸脱氢酶（MDH）、异柠檬酸脱氢酶（ICDH）和乳酸脱氢酶（LDH）活性的昼夜变化。2. 实验小鼠喂食蛋白质含量低（9%）的颗粒玉米饲料，而对照动物则喂食蛋白质含量为25%的标准Murigran颗粒饲料。3. 实验持续7周，动物饲养在光照与黑暗时间比为12:12、温度为24±2摄氏度的环境中。4. 从上午8点开始，每4小时采集一次肝脏样本。5. 两组均观察到AspAT、A1AT、MDH和LDH活性的昼夜节律，而仅在实验动物中发现FDPA活性的昼夜节律。6. 仅在对照小鼠中观察到ICDH活性的昼夜节律。7. 除FDPA和ICDH活性外，低蛋白玉米饲料并未改变所研究酶的节律性。