Cakmak Gokhan, Bolukbasi Selcuk, Simsek Aykin, Erdem Ozlem, Yilmaz Guldal, Senkoylu Alpaslan
Department of Orthopedics and Traumatology, Gazi University School of Medicine, Ankara, Turkey.
Saudi Med J. 2006 Jun;27(6):777-80.
To determine the effect of inorganic bone matric/Pepgen P-15 (ABM/P-15) on the healing of a critical sized segmental defect in a rat radius using a radiological and histological grading system.
We carried out this study at the Research Laboratories, Gazi University School of Medicine in 2004. Critical sized segmental defects were created in the radius of 36 Wistar rats. Thirteen defects were filled with ABM/P-15 Flow (gel form), 12 defects were filled with ABM/P-15, and 11 defects were used as a control group. The rats were sacrificed at the tenth week, and healing of the defects was evaluated radiographically and histologically.
The usage of ABM/P-15 and ABM/P-15 Flow were demonstrated to improve healing of segmental bone defects compared with the control group. Statistical evaluation showed that there were significant differences between control sites, and the sites treated with P-15 and P-15 Flow (p=0.011). The highest radiological and histological grades were achieved by P-15.
Segmental cortical bone defects may be treated with ABM/P-15 instead of bone allografts, and autografts. According to the radiological and histological parameters measured in this study, the implantation of ABM/P-15 resulted in optimum healing of the segmental cortical bone defects. Pepgen P-15 has a positive effect on bone healing, without any immunogenic features and disease transmission risk. Therefore, ABM/P-15 can also be used for orthopedic surgery.
使用放射学和组织学分级系统，确定无机骨基质/佩普基因P - 15（ABM/P - 15）对大鼠桡骨临界尺寸节段性缺损愈合的影响。
我们于2004年在加齐大学医学院研究实验室开展了这项研究。在36只Wistar大鼠的桡骨上制造临界尺寸节段性缺损。13处缺损填充ABM/P - 15流体（凝胶形式），12处缺损填充ABM/P - 15，11处缺损作为对照组。在第10周处死大鼠，通过放射学和组织学评估缺损的愈合情况。
与对照组相比，ABM/P - 15和ABM/P - 15流体的使用被证明可改善节段性骨缺损的愈合。统计学评估显示，对照部位与用P - 15和P - 15流体处理的部位之间存在显著差异（p = 0.011）。P - 15取得了最高的放射学和组织学分级。
节段性皮质骨缺损可用ABM/P - 15替代同种异体骨和自体骨进行治疗。根据本研究中测量的放射学和组织学参数，植入ABM/P - 15可使节段性皮质骨缺损实现最佳愈合。佩普基因P - 15对骨愈合有积极作用，且无任何免疫原性特征和疾病传播风险。因此，ABM/P - 15也可用于骨科手术。
