抗氧化剂在预防紫外线B照射的人HaCaT角质形成细胞中嘧啶二聚体形成中的作用。
Role of antioxidants in prevention of pyrimidine dimer formation in UVB irradiated human HaCaT keratinocytes.
作者信息
Hochberg Malka, Kohen Ron, Enk Claes D
机构信息
Department of Dermatology, Hadassah Medical Center, Hebrew University, Jerusalem, Israel.
出版信息
Biomed Pharmacother. 2006 Jun;60(5):233-7. doi: 10.1016/j.biopha.2006.04.008. Epub 2006 Jun 2.
The objective of the present study was to study the role of reactive oxygen species (ROS) in UVB induced cyclobutane pyrimidine dimer (CPD) formation in human keratinocytes, and to examine the modulating activity of low molecular weight antioxidants. To demonstrate the involvement of ROS, we examined the protective capacity of alpha-tocopherol, tempamine, and diethyldithiocarbamate (DDC) on CPD formation in intact cells and naked DNA. HaCaT cells and naked DNA in water solution were irradiated with UVB in the presence of the antioxidants and CPD was determined by ELISA. We found that all three antioxidants provided protection against UVB induced CPD formation. The protection was observed in intact cells only and not in naked DNA. Since some of the tested antioxidants do not possess UV absorbing qualities, our findings suggest that in a cellular environment ROS play a role in CPD formation.
本研究的目的是探讨活性氧（ROS）在紫外线B（UVB）诱导人角质形成细胞中环丁烷嘧啶二聚体（CPD）形成中的作用，并研究低分子量抗氧化剂的调节活性。为了证明ROS的参与，我们检测了α-生育酚、tempamine和二乙基二硫代氨基甲酸盐（DDC）对完整细胞和裸露DNA中CPD形成的保护能力。在抗氧化剂存在的情况下，用UVB照射HaCaT细胞和水溶液中的裸露DNA，通过酶联免疫吸附测定法（ELISA）测定CPD。我们发现，所有三种抗氧化剂都能提供针对UVB诱导的CPD形成的保护作用。这种保护作用仅在完整细胞中观察到，而在裸露DNA中未观察到。由于一些测试的抗氧化剂不具备紫外线吸收特性，我们的研究结果表明，在细胞环境中ROS在CPD形成中起作用。
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