Badowska-Szalewska E, Ludkiewicz B, Domaradzka-Pytel B, Dziewiatkowski J, Spodnik J H, Moryś J
Department of Anatomy and Neurobiology, Medical University of Gdańsk, Gdańsk, Poland.
Folia Morphol (Warsz). 2006 May;65(2):145-51.
The amygdala is a critical component of the neuroanatomical stress circuit. It plays a role in the generation of responses to emotional stimuli. The central (CeA) and medial (MeA) amygdaloid nuclei are implicated in activation of the hypothalamic-pituitary-adrenocortical (HPA) axis. The immunoreactivity (-ir) of c-Fos, NGF and its receptor, TrkA, following acute and chronic open-field stress were studied in the CeA and MeA nuclei of the amygdala. The material consisted of 21 male adult rats divided into three groups: non-stressed (control) animals, rats exposed to acute (once only lasting 15 min) and chronic (15 min daily over 21 days) aversive stimulation (open-field exposure). The brains were stained with the use of immunohistochemical methods for c-Fos, NGF or TrkA. In the control rats c-Fos-, TrkA- and NGF-ir cells were observed in the nuclei studied, but the quantity varied, being moderate or high (immunoreactive to TrkA and NGF) or low (immunoreactive to c-Fos). In the animals exposed to acute open-field stress the number of c-Fos-ir, NGF-ir and TrkA-ir cells in the nuclei under examination was differentiated but higher than that in the control animals. In the animals exposed to chronic open-field stress the number of c-Fos-ir cells in the nuclei studied was similar and was smaller than those in animals exposed to acute stress. The number of TrkA-ir neurons was also lower in comparison to that in animals exposed to acute stress. However, no significant differences in the number of NGF-ir cells were observed between the groups exposed to acute and chronic stress. Diverse expression of c-Fos protein following both acute and chronic stress stimulation may prove the functional heterogeneity of the amygdaloid nuclei investigated. The decrease observed in both c-Fos- and TrkA-ir in MeA (only TrkA in CeA) of animals exposed to chronic stress may indicate the phenomenon of habituation.
杏仁核是神经解剖学应激回路的关键组成部分。它在对情绪刺激的反应产生中发挥作用。杏仁核的中央核（CeA）和内侧核（MeA）与下丘脑 - 垂体 - 肾上腺皮质（HPA）轴的激活有关。研究了急性和慢性旷场应激后，杏仁核CeA和MeA核中c-Fos、NGF及其受体TrkA的免疫反应性（-ir）。实验材料为21只成年雄性大鼠，分为三组：无应激（对照）动物、暴露于急性（单次持续15分钟）和慢性（21天内每天15分钟）厌恶刺激（旷场暴露）的大鼠。使用免疫组织化学方法对大脑进行c-Fos、NGF或TrkA染色。在对照大鼠中，在所研究的核中观察到了c-Fos-、TrkA-和NGF-ir细胞，但数量有所不同，为中度或高度（对TrkA和NGF免疫反应）或低度（对c-Fos免疫反应）。在暴露于急性旷场应激的动物中，所检查核中的c-Fos-ir、NGF-ir和TrkA-ir细胞数量有差异，但高于对照动物。在暴露于慢性旷场应激的动物中，所研究核中的c-Fos-ir细胞数量相似，且比暴露于急性应激的动物少。与暴露于急性应激的动物相比，TrkA-ir神经元的数量也更低。然而，在暴露于急性和慢性应激的组之间，NGF-ir细胞数量没有观察到显著差异。急性和慢性应激刺激后c-Fos蛋白的不同表达可能证明了所研究的杏仁核核团的功能异质性。在暴露于慢性应激的动物的MeA（CeA中仅TrkA）中观察到的c-Fos-和TrkA-ir的减少可能表明了习惯化现象。
Zotero 插件