Miksík Ivan, Sedláková Pavla, Mikulíková Katerina, Eckhardt Adam, Cserhati Tibor, Horváth Tibor
Institute of Physiology, Academy of Sciences of the Czech Republic, Prague.
Biomed Chromatogr. 2006 Jun-Jul;20(6-7):458-65. doi: 10.1002/bmc.640.
This article gives an overview of uncommon replaceable matrices (gels) for capillary gel electrophoresis. This electrophoretic technique is useful mainly for the separation and analysis of biopolymers-nucleic acids and their fragments, and proteins/peptides. Commonly used gels are not reviewed. Those mentioned and discussed here are gels containing saccharides, newly developed acrylamide-based gels and thermoadjustable viscosity polymers, namely triblock copolymers and grafted polyacrylamide.
本文概述了用于毛细管凝胶电泳的罕见可替换基质(凝胶)。这种电泳技术主要用于生物聚合物——核酸及其片段、蛋白质/肽的分离和分析。本文不讨论常用的凝胶。这里提及并讨论的凝胶包括含糖类凝胶、新开发的基于丙烯酰胺的凝胶以及热调节粘度聚合物,即三嵌段共聚物和接枝聚丙烯酰胺。