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毛细管凝胶电泳基质——罕见凝胶的简要概述

Matrices for capillary gel electrophoresis--a brief overview of uncommon gels.

作者信息

Miksík Ivan, Sedláková Pavla, Mikulíková Katerina, Eckhardt Adam, Cserhati Tibor, Horváth Tibor

机构信息

Institute of Physiology, Academy of Sciences of the Czech Republic, Prague.

出版信息

Biomed Chromatogr. 2006 Jun-Jul;20(6-7):458-65. doi: 10.1002/bmc.640.

Abstract

This article gives an overview of uncommon replaceable matrices (gels) for capillary gel electrophoresis. This electrophoretic technique is useful mainly for the separation and analysis of biopolymers-nucleic acids and their fragments, and proteins/peptides. Commonly used gels are not reviewed. Those mentioned and discussed here are gels containing saccharides, newly developed acrylamide-based gels and thermoadjustable viscosity polymers, namely triblock copolymers and grafted polyacrylamide.

摘要

本文概述了用于毛细管凝胶电泳的罕见可替换基质(凝胶)。这种电泳技术主要用于生物聚合物——核酸及其片段、蛋白质/肽的分离和分析。本文不讨论常用的凝胶。这里提及并讨论的凝胶包括含糖类凝胶、新开发的基于丙烯酰胺的凝胶以及热调节粘度聚合物,即三嵌段共聚物和接枝聚丙烯酰胺。

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