Shamekh Rania, Mallery Jennifer, Newcomb Jennifer, Hushen Joelle, Saporta Samuel, Cameron Don F, Sanberg Cyndy Davis, Sanberg Paul R, Willing Alison E
Department of Neurosurgery, Center of Excellence for Aging and Brain Repair, University of South Florida, Tampa, 33612, USA.
Brain Res. 2006 Jun 22;1096(1):1-10. doi: 10.1016/j.brainres.2006.04.058. Epub 2006 Jun 15.
Sertoli cells (SCs) are testis-derived cells that secrete trophic factors important for the development of germ cells. Both porcine and rat SCs have been used as graft facilitators - neonatal porcine SCs to support islets in diabetes and 15-day-old rat SCs to enhance dopaminergic neuron transplants in Parkinson's disease models. However, there has never been a study examining the optimal SCs preparation to enhance tyrosine hydroxylase expression in the ventral mesencephalon (VM) neuron. The aim of this study was to compare the ability of both rat and porcine SCs to enhance tyrosine hydroxylase expression (TH) and neuronal survival at the same postnatal developmental ages. The SCs were isolated from 1-, 9-, or 15-day-old rat, or neonate (2-5 days), 2-month, or 4-month-old pig, and co-cultured with VM tissue from 13.5-day-old embryos. Our results showed that VM neurons co-cultured with SCs dispersed over the culture plate and had extensive neuritic outgrowth, while VM neurons cultured alone tended to cluster together forming a mass of cells with limited neurite outgrowth. TH expression was significantly increased when VM neurons were co-cultured with 15-day rat SCs or 2-month pig SCs but not when the cells were co-cultured with other ages of SCs. This suggests that secretion of trophic factors by SCs varies according to the developmental age, and it is critical for the success of graft facilitation that SCs from the appropriate age and species be used.
支持细胞（SCs）是源自睾丸的细胞，可分泌对生殖细胞发育至关重要的营养因子。猪和大鼠的支持细胞都已被用作移植促进剂——新生猪的支持细胞用于支持糖尿病中的胰岛，15日龄大鼠的支持细胞用于增强帕金森病模型中多巴胺能神经元的移植。然而，从未有过研究探讨增强腹侧中脑（VM）神经元中酪氨酸羟化酶表达的最佳支持细胞制备方法。本研究的目的是比较大鼠和猪支持细胞在相同出生后发育年龄增强酪氨酸羟化酶表达（TH）和神经元存活的能力。支持细胞分别从1日龄、9日龄或15日龄的大鼠，或新生（2 - 5日龄）、2月龄或4月龄的猪中分离出来，并与13.5日龄胚胎的VM组织共培养。我们的结果表明，与分散在培养板上的支持细胞共培养的VM神经元有广泛的神经突生长，而单独培养的VM神经元倾向于聚集在一起形成一团细胞，神经突生长有限。当VM神经元与15日龄大鼠支持细胞或2月龄猪支持细胞共培养时，TH表达显著增加，但与其他年龄的支持细胞共培养时则没有增加。这表明支持细胞分泌的营养因子随发育年龄而变化，使用适当年龄和物种的支持细胞对于移植促进的成功至关重要。
