从牛奶厂废弃物中分离出的铜绿假单胞菌B189产生的鼠李糖脂的化学结构和生物活性。
Chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste.
作者信息
Thanomsub Benjamas, Pumeechockchai Wanna, Limtrakul Anirut, Arunrattiyakorn Panarat, Petchleelaha Wipawan, Nitoda Teruhiko, Kanzaki Hiroshi
机构信息
Department of Microbiology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110, Thailand.
出版信息
Bioresour Technol. 2007 Mar;98(5):1149-53. doi: 10.1016/j.biortech.2005.10.045. Epub 2006 Jun 14.
The aim of this work was to study chemical structures and biological activities of rhamnolipids produced by Pseudomonas aeruginosa B189 isolated from milk factory waste. The culture produced two biosurfactants, a and b, which showed strong activity and were identified as L-rhamnopyranosyl-L-rhamnopyranosyl-beta-hydroxydecanoyl-beta-hydroxydecanoate or Rha-Rha-C10-C10 and L-rhamnopyranosyl-L-rhamnopyranosyl-beta-hydroxydecanoyl-beta-hydroxydodecanoate or Rha-Rha-C10-C12, respectively. Both compounds exhibited higher surfactant activities tested by the drop collapse test than several artificial surfactants such as SDS and Tween 80. Rhamnolipid a showed significant antiproliferative activity against human breast cancer cell line (MCF-7) at minimum inhibitory concentration (MIC) at 6.25 microg/mL while rhamnolipid b showed MIC against insect cell line C6/36 at 50 microg/mL.
这项工作的目的是研究从牛奶厂废料中分离出的铜绿假单胞菌B189产生的鼠李糖脂的化学结构和生物活性。该培养物产生了两种生物表面活性剂，a和b，它们表现出很强的活性，分别被鉴定为L-鼠李吡喃糖基-L-鼠李吡喃糖基-β-羟基癸酰基-β-羟基癸酸酯或Rha-Rha-C10-C10和L-鼠李吡喃糖基-L-鼠李吡喃糖基-β-羟基癸酰基-β-羟基十二酸酯或Rha-Rha-C10-C12。通过液滴崩塌试验测试，这两种化合物都表现出比几种人工表面活性剂如SDS和吐温80更高的表面活性剂活性。鼠李糖脂a在最低抑菌浓度（MIC）为6.25μg/mL时对人乳腺癌细胞系（MCF-7）表现出显著的抗增殖活性，而鼠李糖脂b在50μg/mL时对昆虫细胞系C6/36表现出MIC。
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