Gao Chunli, Stading Mats, Wellner Nikolaus, Parker Mary L, Noel Timothy R, Mills E N Clare, Belton Peter S
Institute of Food Research, Colney Lane, Norwich NR4 7UA, United Kingdom.
J Agric Food Chem. 2006 Jun 28;54(13):4611-6. doi: 10.1021/jf060611w.
Kafirin, the seed storage protein of the cereal sorghum, is highly homologous with the maize storage protein zein. The effects of plasticisation of a kafirin film by glycerol in the absence of water were examined by a combination of spectroscopic (NMR and infrared), rheological, and calorimetric methods. The results suggest that at low glycerol levels the glycerol is absorbed onto and possibly into the protein. Increasing the level of glycerol increases the motion of the protein and changes the protein conformation. There are corresponding changes of the mechanical properties of protein films. At 40% (w/w) of glycerol, two glass transition temperatures were observed, one of which corresponded to the glass transition temperature of pure glycerol. This result indicates that at this level of plasticizer there are sufficient glycerol/glycerol interactions occurring to allow a separate glass formation process for glycerol.
高粱醇溶蛋白是谷物高粱的种子贮藏蛋白，与玉米贮藏蛋白玉米醇溶蛋白高度同源。通过光谱法（核磁共振和红外光谱）、流变学法和量热法相结合的方式，研究了在无水条件下甘油对高粱醇溶蛋白膜的增塑作用。结果表明，在甘油含量较低时，甘油被吸附到蛋白质上，甚至可能进入蛋白质内部。甘油含量的增加会增强蛋白质的运动并改变蛋白质的构象。蛋白质膜的机械性能也会相应发生变化。当甘油含量为40%（w/w）时，观察到两个玻璃化转变温度，其中一个与纯甘油的玻璃化转变温度相对应。这一结果表明，在该增塑剂含量水平下，甘油/甘油之间存在足够的相互作用，从而使得甘油能够形成独立的玻璃态。
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