[一氧化碳和一氧化氮对高热惊厥大鼠海马神经元凋亡的影响]

[Effect of carbon monoxide and nitric oxide on the apoptosis of hippocampal neurons in rats with febrile seizures].

作者信息

Yang Zhi-Xian, Qin Jiong, Chang Xing-Zhi, Han Ying, Shan Ying

机构信息

Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.

出版信息

Zhongguo Dang Dai Er Ke Za Zhi. 2006 Jun;8(3):231-4.

PMID:16787598

Abstract

OBJECTIVE

Febrile seizure (FS) is the most common type of seizure disorders in children. Recurrent FS can cause hippocampal neurons injury. At the same time heme oxygenase/carbon monoxide (HO/CO) system and nitric oxide synthase/nitric oxide (NOS/NO) system were up-regulated and interacted each other. This study examined the effects of the two systems on the apoptosis of hippocampal neurons in rats with recurrent FS.

METHODS

FS was induced in rats by exposure to warm water bath (45.2 degrees C), once every 2 days, 10 times in all. Sprague-Dawley (SD) rats aged 21 days were randomly assigned into four groups: Control (37 degrees C water bath exposure), FS, FS + ZnPP-IX (HO inhibitor) and FS + L-NAME (NOS inhibitor) groups. The apoptosis of hippocampal CA1 neurons was detected by TUNEL.

RESULTS

After recurrent FS, the apoptotic cells in the hippocampal CA1 neurons increased by 225% compared with those in the Control group (P < 0.01). The apoptotic cells in the FS+ZnPP-IX group increased by 62% and 425% compared with those in the FS and the Control groups (both P < 0.01). The apoptotic cells in the FS + L-NAME group decreased by 38% compared with those in the FS group (P < 0.01) and increased by 100% compared with those in the Control group (P < 0.05).

CONCLUSIONS

In recurrent FS, exogenous administration of HO inhibitor ZnPP-IX may induce an increase of apoptotic cells in hippocampal neurons, while NOS inhibitor L-NAME may decrease the apoptotic cells. The results suggest that the HO/CO system might alleviate neuronal damage, while NOS/NO system might augment neuronal damage.

摘要

目的

热性惊厥（FS）是儿童最常见的惊厥性疾病类型。复发性FS可导致海马神经元损伤。同时，血红素氧合酶/一氧化碳（HO/CO）系统和一氧化氮合酶/一氧化氮（NOS/NO）系统上调并相互作用。本研究探讨这两个系统对复发性FS大鼠海马神经元凋亡的影响。

方法

通过温水浴（45.2℃）诱导大鼠发生FS，每2天诱导1次，共诱导10次。将21日龄的Sprague-Dawley（SD）大鼠随机分为四组：对照组（37℃水浴暴露）、FS组、FS + ZnPP-IX（HO抑制剂）组和FS + L-NAME（NOS抑制剂）组。采用TUNEL法检测海马CA1区神经元凋亡情况。

结果

复发性FS后，海马CA1区神经元凋亡细胞数较对照组增加225%（P < 0.01）。FS + ZnPP-IX组凋亡细胞数较FS组和对照组分别增加62%和425%（均P < 0.01）。FS + L-NAME组凋亡细胞数较FS组减少38%（P < 0.01），较对照组增加100%（P < 0.05）。