Dutta Anirban, Singh Shashi Kr, Ghosh Payel, Mukherjee Runni, Mitter Sayak, Bandyopadhyay Debashis
Department of Biotechnology, Bengal College of Engineering & Technology, Durgapur 713 212, India.
In Silico Biol. 2006;6(1-2):43-7.
Availability of genome sequences of pathogens has provided a tremendous amount of information that can be useful in drug target and vaccine target identification. One of the recently adopted strategies is based on a subtractive genomics approach, in which the subtraction dataset between the host and pathogen genome provides information for a set of genes that are likely to be essential to the pathogen but absent in the host. This approach has been used successfully in recent times to identify essential genes in Pseudomonas aeruginosa. We have used the same methodology to analyse the whole genome sequence of the human gastric pathogen Helicobacter pylori. Our analysis revealed that out of the 1590 coding sequences of the pathogen, 40 represent essential genes that have no human homolog. We have further analysed these 40 genes by the protein sequence databases to list some 10 genes whose products are possibly exposed on the pathogen surface. This preliminary work reported here identifies a small subset of the Helicobacter proteome that might be investigated further for identifying potential drug and vaccine targets in this pathogen.
病原体基因组序列的可得性提供了大量信息，这些信息在药物靶点和疫苗靶点识别中可能有用。最近采用的一种策略基于消减基因组学方法，其中宿主和病原体基因组之间的消减数据集为一组可能对病原体至关重要但在宿主中不存在的基因提供了信息。这种方法最近已成功用于鉴定铜绿假单胞菌中的必需基因。我们使用相同的方法分析了人类胃病原体幽门螺杆菌的全基因组序列。我们的分析表明，在该病原体的1590个编码序列中，有40个代表没有人类同源物的必需基因。我们通过蛋白质序列数据库进一步分析了这40个基因，列出了约10个其产物可能暴露在病原体表面的基因。此处报道的这项初步工作确定了幽门螺杆菌蛋白质组的一个小亚组，可能需要进一步研究以确定该病原体中的潜在药物和疫苗靶点。
