Monitoring DNA replication following UV-induced damage in Escherichia coli.

The question of how the replication machinery accurately copies the genomic template in the presence of DNA damage has been intensely studied for more than forty years. A large number of genes has been characterized that, when mutated, are known to impair the ability of the cell to replicate in the presence of DNA damage. This chapter describes three techniques that can be used to monitor the progression, degradation, and structural properties of replication forks following UV-induced DNA damage in Escherichia coli.