次氯酸盐（HOCl）修饰的低密度脂蛋白和高密度脂蛋白对血小板功能的影响。

Effect of hypochlorite (HOCl)-modified low density lipoproteins and high density lipoproteins on platelet function.

作者信息

Opper C, Schüssler G, Sattler W, Malle E

机构信息

Philipps University, Institute of Physiological Chemistry, Marburg/Lahn, Germany.

出版信息

Platelets. 1998;9(5):339-41. doi: 10.1080/09537109876582.

DOI:10.1080/09537109876582

PMID:16793758

Abstract

As hypochlorous acid (HOCl) might be a potential candidate for generation of modified (lipo)proteins in vivo , the present study was aimed at investigating the effects of HOCl-modified lipoproteins on platelet function in vitro. Lipoproteins modified with HOCl at concentrations that occur physiologically did not induce spontaneous platelet aggregation. However, low density lipoproteins (LDL; 100 to 500 microg protein/ml) increased platelet aggregation and fluorescence anisotropy in response to ADP (1 and 10 microM) and thrombin (0.1 and 0.5 U/ml) as well as malondialdehyde (MDA) formation as a function of increasing HOCl-concentrations (0.2 to 1.6 mM). HOCl-modified high density lipoprotein subfraction 3 (HDL3) had no effect on platelet function.

摘要

由于次氯酸（HOCl）可能是体内生成修饰（脂蛋白）的潜在候选物质，本研究旨在体外研究HOCl修饰的脂蛋白对血小板功能的影响。在生理浓度下用HOCl修饰的脂蛋白不会诱导血小板自发聚集。然而，低密度脂蛋白（LDL；100至500微克蛋白质/毫升）随着HOCl浓度（0.2至1.6毫摩尔）的增加，对ADP（1和10微摩尔）、凝血酶（0.1和0.5单位/毫升）以及丙二醛（MDA）形成的反应增加了血小板聚集和荧光各向异性。HOCl修饰的高密度脂蛋白亚组分3（HDL3）对血小板功能没有影响。