Ali Rehab E, Rattan Suresh I S
Laboratory of Cellular Ageing, Danish Centre for Molecular Gerontology, Department of Molecular Biology, University of Aarhus, Denmark.
Ann N Y Acad Sci. 2006 May;1067:394-9. doi: 10.1196/annals.1354.056.
Curcumin (diferuloylmethane), is a component of the yellow powder prepared from the roots of Curcuma longa (Zingiberaceae), also known as tumeric or turmeric. It is widely cultivated and used as a food ingredient in tropical areas of Asia and Central America. Treatment of mid-passage human epidermal keratinocytes with curcumin resulted in a biphasic hormetic dose-response with respect to proteasome activity. Curcumin treatment (up to 1 microM for 24 h) increased chymotrypsin-like activity by 46% compared to that in untreated keratinocytes. However, higher concentrations of curcumin were inhibitory, and at 10 microM the proteasome activity decreased to 46% of its initial value. Furthermore, the preincubation of human keratinocytes at 43 degrees C for 1 h, followed by 24-h treatment with 3 microM curcumin, led to an increase in heat-shock protein (hsp70 and hsp90) levels by 24% and 19%, respectively, and the effect was sustained at concentrations up to 10 microM. On the other hand, the level of the small hsp27 was unaffected by curcumin concentrations of 0.3-1 microM, while it decreased by 34% at 10 microM.
姜黄素(二阿魏酰甲烷)是从姜黄(姜科)的根中制备的黄色粉末的一种成分,姜黄也被称为姜黄根粉或姜黄。它在亚洲和中美洲的热带地区广泛种植并用作食品成分。用姜黄素处理传代中期的人表皮角质形成细胞,就蛋白酶体活性而言,产生了双相性的 hormetic 剂量反应。与未处理的角质形成细胞相比,姜黄素处理(高达 1 microM,处理 24 小时)使胰凝乳蛋白酶样活性提高了 46%。然而,更高浓度的姜黄素具有抑制作用,在 10 microM 时,蛋白酶体活性降至其初始值的 46%。此外,如果先将人角质形成细胞在 43 摄氏度下预孵育 1 小时,然后用 3 microM 姜黄素处理 24 小时,热休克蛋白(hsp70 和 hsp90)水平分别提高 24%和 19%,并且在高达 10 microM 的浓度下该效果仍然存在。另一方面,小 hsp27 的水平在 0.3 - 1 microM 的姜黄素浓度下不受影响,而在 10 microM 时下降了 34%。