在测定总碳水化合物缺乏转铁蛋白百分比方面，毛细管电泳与免疫测定法的新型比较
Novel comparison of capillary electrophoresis versus immunoassay in the measurement of total percent carbohydrate deficient transferrin.
作者信息
Strauss Kathy A, O'Kane Jessica, Cook Janine Denis
机构信息
Department of Epidemiology and Preventive Medicine, University of Maryland, Baltimore, Baltimore, MD 21201, USA.
出版信息
Clin Biochem. 2006 Aug;39(8):826-32. doi: 10.1016/j.clinbiochem.2006.04.022. Epub 2006 May 19.
OBJECTIVES
Novel comparison of CDT isoforms as determined by CE with an FDA-approved immunoassay kit.
DESIGN AND METHODS
Subjects (n=51) were categorized by drinking status based on AUDIT questionnaire responses. CDT isoform analyses by CE were compared to a commercially available, FDA-approved immunoassay. The analytical specificity of the immunoassay kit was assessed by analysis with IEF.
RESULTS
Because of the poor correlation between % CDT as measured by CE and the TIA immunoassay and between subject-reported drinking levels and results from the TIA assay, extraction column eluants from the immunoassays were analyzed by IEF for analytical specificity. % CDT by TIA included some trisialo-Tf, a non-CDT fraction, in the % CDT determination.
CONCLUSIONS
Total % CDT by CE, which separates all isoforms is more analytically specific than immunoassays because it does not include trisialo-Tf in the CDT calculation.
目的
通过毛细管电泳（CE）测定的CDT同工型与美国食品药品监督管理局（FDA）批准的免疫分析试剂盒之间的新型比较。
设计与方法
根据酒精使用障碍识别测试（AUDIT）问卷的回答，将受试者（n = 51）按饮酒状况进行分类。将通过CE进行的CDT同工型分析与市售的、FDA批准的免疫分析进行比较。通过等电聚焦（IEF）分析评估免疫分析试剂盒的分析特异性。
结果
由于CE测定的CDT百分比与TIA免疫分析之间以及受试者报告的饮酒水平与TIA分析结果之间的相关性较差，因此通过IEF分析免疫分析的萃取柱洗脱液的分析特异性。TIA测定的CDT百分比在CDT测定中包括一些三唾液酸转铁蛋白（trisialo-Tf），这是一种非CDT组分。
结论
通过CE测定的总CDT百分比可分离所有同工型，在分析上比免疫分析更具特异性，因为它在CDT计算中不包括三唾液酸转铁蛋白。
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