[通过原子力显微镜对大肠杆菌和海栖热袍菌核糖体的研究]

[Investigation of ribosomes of E. coli and T. maritima by atomic force microscopy].

作者信息

Maliuchenko N V, Tonevitskiĭ E A, Agapov I I, Pevzner I B, Bykov V A, Kirpichnikov M P, Tonevitskiĭ A G

出版信息

Biofizika. 2006 May-Jun;51(3):440-5.

Abstract

Subunits 70S, 50S, and 30S of ribosomes of E. coli and T. maritima have been studied by atomic force microscopy. A considerable heterogeneity of structures was visualized when 70S and 30S subunits were sorbed on mica. The linear size and the height of molecules were estimated. It was found that the heights of ribosomes of E. coli and T. maritima substantially differ. The average height of 70S ribosomes of E. coli was 9.4 + 0.01 nm and that of T. maritima was 10.35 +/- 0.02 nm. The differences in the dimensions were probably determined by special organization of the mobile ribosomal element the L7/L12-stalk.

摘要

利用原子力显微镜对大肠杆菌和海栖热袍菌核糖体的70S、50S和30S亚基进行了研究。当70S和30S亚基吸附在云母上时，可以看到结构存在相当大的异质性。估计了分子的线性尺寸和高度。发现大肠杆菌和海栖热袍菌核糖体的高度有显著差异。大肠杆菌70S核糖体的平均高度为9.4 + 0.01纳米，海栖热袍菌的平均高度为10.35 +/- 0.02纳米。尺寸上的差异可能是由核糖体移动元件L7/L12柄的特殊组织决定的。