Donnerer J
Department of Experimental and Clinical Pharmacology, University of Graz, Austria.
Brain Res. 1991 Aug 2;555(2):332-4. doi: 10.1016/0006-8993(91)90360-8.
A release of endogenous glutamate (Glu), aspartate (Asp) and gamma-aminobutyric acid (GABA) from rat dorsal spinal cord slices was evoked by a 60 mM K(+)-depolarization (calcium dependent) and by 50 microM veratridine (tetrodotoxin sensitive) indicating a neuronal exocytosis mechanism. No release of the 3 amino acids investigated was obtained with the selective C-fiber excitant capsaicin (1 microM) and after impairment of afferent C-fibers the amount of amino acids released by K(+)-depolarization or veratridine was not reduced. These results suggest that amino acids may be exclusively transmitters of capsaicin-insensitive nerve structures in the rat dorsal spinal cord.
60 mM钾离子去极化（依赖钙）和50微摩尔藜芦碱（对河豚毒素敏感）可诱发大鼠背脊髓切片释放内源性谷氨酸（Glu）、天冬氨酸（Asp）和γ-氨基丁酸（GABA），这表明存在神经元胞吐机制。用选择性C纤维兴奋剂辣椒素（1微摩尔）未观察到所研究的3种氨基酸的释放，并且在传入C纤维受损后，钾离子去极化或藜芦碱释放的氨基酸量并未减少。这些结果表明，氨基酸可能仅是大鼠背脊髓中对辣椒素不敏感的神经结构的递质。
