Munyard K A, Baker S K
CSIRO Livestock Industries, Floreat Park Laboratories, Wembley, Western Australia, Australia.
J Microbiol Methods. 2006 Dec;67(3):566-73. doi: 10.1016/j.mimet.2006.05.008. Epub 2006 Jul 3.
The microorganisms in rumen contents were physically separated into five fractions on the basis of size using counter-flow centrifugal elutriation (CCE). The use of CCE allowed the microbial population to be separated in a highly repeatable manner into two protozoal and three bacterial fractions with minimal loss of material (dry weight), and with no visible damage to the microorganisms. A Coulter counter was used to determine the sizes of the organisms in each fraction. The modified CCE method is suitable for studies of the rumen microbial ecosystem that require separation of defined fractions of the population.
使用逆流离心淘析法（CCE），根据大小将瘤胃内容物中的微生物物理分离为五个部分。CCE的使用能够以高度可重复的方式将微生物群体分离为两个原生动物部分和三个细菌部分，物质（干重）损失最小，且微生物无明显损伤。使用库尔特计数器来确定每个部分中生物体的大小。改良的CCE方法适用于需要分离特定群体部分的瘤胃微生物生态系统研究。
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