一个编码序列相似性家族84成员A（FAM84A）的基因增强了人结肠癌细胞的迁移能力。
A gene encoding a family with sequence similarity 84, member A (FAM84A) enhanced migration of human colon cancer cells.
作者信息
Kobayashi Takaaki, Masaki Tadahiko, Sugiyama Masanori, Atomi Yutaka, Furukawa Yoichi, Nakamura Yusuke
机构信息
Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan.
出版信息
Int J Oncol. 2006 Aug;29(2):341-7.
Using genome-wide cDNA microarray analysis, we identified a number of genes whose expression was up-regulated frequently in colorectal cancer. One of these was a gene termed FAM84A that was not expressed in any of the 23 normal tissues examined except the testis. Although immuno-cytochemical staining revealed localization of FAM84A protein in the subcellular membrane region, the staining was limited to the region lacking attachment with neighboring cells. In addition, we found that exogenous FAM84A expression increased cell motility in NIH3T3 cells, and that phosphorylation of serine 38 of FAM84A was associated with morphology of cells. Our results indicate a possibility that up-regulation of FAM84A plays a critical role in progression of colon cancer.
通过全基因组cDNA微阵列分析，我们鉴定出了一些在结直肠癌中表达经常上调的基因。其中之一是一个名为FAM84A的基因，在除睾丸外的23种正常组织中均未表达。尽管免疫细胞化学染色显示FAM84A蛋白定位于亚细胞膜区域，但染色仅限于与相邻细胞缺乏附着的区域。此外，我们发现外源性FAM84A表达增加了NIH3T3细胞的运动性，并且FAM84A丝氨酸38位的磷酸化与细胞形态有关。我们的结果表明，FAM84A的上调可能在结肠癌进展中起关键作用。
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