Yedjou Clement G, Moore Pamela, Tchounwou Paul B
Molecular Toxicology Research Laboratory, NIH-RCMI Center for Environmental Health, College of Science, Engineering and Technology, Jackson State University, 1400 Lynch Street, P.O. Box 18540, Jackson, Mississippi, USA.
Int J Environ Res Public Health. 2006 Jun;3(2):136-40. doi: 10.3390/ijerph2006030017.
The treatment of acute promyelocytic leukemia (APL) has been based on the administration of all-trans retinoic acid plus anthracycline chemotherapy, which is very effective as first line therapy; however 25 to 30% of patients will relapse with their disease becoming refractory to conventional therapy. Recently, studies have shown arsenic trioxide to be effective in the treatment of acute promyelocytic leukemia. In this study, we used the human leukemia (HL-60) cell line as a model to evaluate the cytoxicity of arsenic trioxide based on the MTT assay. Data obtained from this assay indicated that arsenic trioxide significantly reduced the viability of HL-60 cells, showing LD50 values of 14.26 + 0.5microg/mL, 12.54 + 0.3microg/mL, and 6.4 + 0.6microg/mL upon 6, 12, and 24 hours of exposure, respectively; indicating a dose- and time-dependent response relationship. Findings from the present study indicate that arsenic trioxide is highly cytotoxic to human leukemia (HL-60) cells, supporting its use as an effective therapeutic agent in the management of acute promyelocytic leukemia.
急性早幼粒细胞白血病（APL）的治疗一直基于全反式维甲酸联合蒽环类化疗药物，作为一线治疗非常有效；然而，25%至30%的患者会复发，其疾病会对传统治疗产生耐药性。最近，研究表明三氧化二砷对急性早幼粒细胞白血病的治疗有效。在本研究中，我们以人白血病（HL-60）细胞系为模型，基于MTT法评估三氧化二砷的细胞毒性。该试验获得的数据表明，三氧化二砷显著降低了HL-60细胞的活力，在分别暴露6小时、12小时和24小时后，LD50值分别为14.26±0.5μg/mL、12.54±0.3μg/mL和6.4±0.6μg/mL；表明存在剂量和时间依赖性反应关系。本研究结果表明，三氧化二砷对人白血病（HL-60）细胞具有高度细胞毒性，支持其作为急性早幼粒细胞白血病治疗的有效治疗剂。
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