Rodríguez Tamara, Pérez Oliver a, Ugrinovic Sanja, Bracho Gustavo, Mastroeni Pietro
Department of Immunology, Finlay Institute, 27 Ave, b/198 and 202 La Lisa, P.O. Box 16017, Havana, Cuba.
Vaccine. 2006 Apr 12;24 Suppl 2:S2-24-5. doi: 10.1016/j.vaccine.2005.01.106.
We explored the potential of a proteoliposome (PL) from the outer membrane of N. meningitidis B, as an immunopotentiator and as a vector for antigen delivery to dendritic cells (DC). DC were incubated with PL resulting in up-regulation of MHC-II, CD40, CD80, and CD86 expression and production of TNFalpha and IL12(p70). Ovoalbumin (OVA) was incorporated within PL (PL-OVA). PL-OVA presented OVA-specific peptides to CD4+ and CD8+ OVA-specific T-cell hybridomas. PL exerts an immunomodulatory effect on DC and is a general system to deliver antigens for presentation to CD4+ and CD8+ T-cells possibly implicated in the induction CD8+ cytotoxic T lymphocytes (CTLs) responses.
我们探究了来自B群脑膜炎奈瑟菌外膜的蛋白脂质体（PL）作为免疫增强剂以及作为将抗原递送至树突状细胞（DC）的载体的潜力。将DC与PL一起孵育，导致MHC-II、CD40、CD80和CD86表达上调以及TNFα和IL12（p70）的产生。卵清蛋白（OVA）被包入PL（PL-OVA）中。PL-OVA将OVA特异性肽呈递给CD4⁺和CD8⁺OVA特异性T细胞杂交瘤。PL对DC发挥免疫调节作用，并且是一种将抗原递呈给CD4⁺和CD8⁺T细胞的通用系统，这可能与诱导CD8⁺细胞毒性T淋巴细胞（CTL）反应有关。
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