[基于RhD阴性母亲血浆中胎儿DNA的胎儿RhD血型产前测定——二次发表]

[Antenatal determination of fetal RhD-blood type based on fetal DNA in plasma from the RhD-negative mother--secondary publication].

作者信息

Clausen Frederik Banch, Krog Grethe Risum, Rieneck Klaus, Nielsen Leif Kofoed, Lundquist Rasmus, Finning Kirsten, Dickmeiss Ebbe, Hedegaard Morten, Dziegiel Morten Hanefeld

机构信息

H:S Rigshospitalet, H:S Blodbank, KI 2034, DK-2100 København Ø.

出版信息

Ugeskr Laeger. 2006 Jun 26;168(26-32):2568-70.

PMID:16824414

Abstract

We present a reliable test for prenatal prediction of fetal RhD type using maternal plasma from RhD-women. This test is needed for a future antenatal RH prophylaxis. A new real time PCR based assay targeting RHD exon 7 and a published assay for RHD exon 10 were used to determine the fetal RHD status in DNA extracted from plasma from 56 pregnant women in 15th-36th week of gestation. Prediction of fetal RhD type was compared with the phenotype determined after birth, and showed 100% concordance. This setup will be of value in antenatal RH prophylaxis and in the management of immunised women.

摘要

我们提出了一种可靠的检测方法，用于利用RhD阴性孕妇的母血血浆对胎儿RhD血型进行产前预测。未来的产前Rh预防需要这种检测方法。我们使用一种针对RHD外显子7的新型实时荧光定量PCR检测方法以及一种已发表的针对RHD外显子10的检测方法，来确定从妊娠第15至36周的56名孕妇血浆中提取的DNA的胎儿RHD状态。将胎儿RhD血型的预测结果与出生后确定的表型进行比较，结果显示二者完全一致。这种检测方法在产前Rh预防以及免疫妇女的管理中将具有重要价值。