Arata Yukinobu, Kouike Hiroko, Zhang Yanping, Herman Michael A, Okano Hideyuki, Sawa Hitoshi
Laboratory for Cell Fate Decision, RIKEN, Center for Developmental Biology, Kobe 650-0047, Japan.
Dev Cell. 2006 Jul;11(1):105-15. doi: 10.1016/j.devcel.2006.04.020.
Asymmetric cell division is a mechanism for achieving cellular diversity. In C. elegans, many asymmetric cell divisions are controlled by the Wnt-MAPK pathway through POP-1/TCF. It is poorly understood, however, how POP-1 determines the specific fates of daughter cells. We found that nob-1/Hox, ceh-20/Pbx, and a Meis-related gene, psa-3, are required for asymmetric division of the T hypodermal cell. psa-3 expression was asymmetric between the T cell daughters, and it was regulated by POP-1 through a POP-1 binding site in the psa-3 gene. psa-3 expression was also regulated by NOB-1 and CEH-20 through a NOB-1 binding sequence in a psa-3 intron. PSA-3 can bind CEH-20 and function after the T cell division to promote the proper fate of the daughter cell. These results indicate that cooperation between Wnt signaling and a Hox protein functions to determine the specific fate of a daughter cell.
不对称细胞分裂是实现细胞多样性的一种机制。在秀丽隐杆线虫中，许多不对称细胞分裂由Wnt-MAPK信号通路通过POP-1/TCF进行调控。然而，人们对POP-1如何决定子细胞的特定命运了解甚少。我们发现，nob-1/Hox、ceh-20/Pbx以及一个与Meis相关的基因psa-3，是T型皮下细胞不对称分裂所必需的。psa-3在T细胞的子细胞之间呈不对称表达，并且它通过psa-3基因中的一个POP-1结合位点受POP-1调控。psa-3的表达还通过psa-3内含子中的一个NOB-1结合序列受NOB-1和CEH-20调控。PSA-3可以结合CEH-20，并在T细胞分裂后发挥作用，以促进子细胞的正常命运。这些结果表明，Wnt信号与一种Hox蛋白之间的协同作用决定了子细胞的特定命运。
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