Ioannidis Nikolaos E, Sfichi Liliana, Kotzabasis Kiriakos
Department of Biology, University of Crete, Heraklion, Greece.
Biochim Biophys Acta. 2006 Jul;1757(7):821-8. doi: 10.1016/j.bbabio.2006.05.034. Epub 2006 May 27.
Putrescine is a main polyamine found in animals, plants and microbes, but the molecular mechanism underlying its mode of action is still obscure. In vivo chlorophyll a fluorescence in tobacco leaf discs indicated that putrescine treatment affects the energization of the thylakoid membrane. Molecular dissection of the electron transport chain by biophysical and biochemical means provided new evidence that putrescine can play an important bioenergetic role acting as a cation and as a permeant natural buffer. We demonstrate that putrescine increases chemiosmotic ATP synthesis more than 70%. Also a regulation of the energy outcome by small changes in putrescine pool under the same photonic environment (i.e., photosynthetically active radiation) is shown. The proposed molecular mechanism has at least four conserved features: (i) presence of a membrane barrier, (ii) a proton-driven ATPase, (iii) a DeltapH and (iv) a pool of putrescine.
腐胺是在动物、植物和微生物中发现的一种主要多胺，但其作用方式的分子机制仍不清楚。烟草叶圆片中的体内叶绿素a荧光表明，腐胺处理会影响类囊体膜的能量供应。通过生物物理和生化手段对电子传递链进行分子剖析提供了新的证据，表明腐胺可以作为阳离子和渗透性天然缓冲剂发挥重要的生物能量作用。我们证明，腐胺可使化学渗透ATP合成增加70%以上。在相同光子环境（即光合有效辐射）下，腐胺池的微小变化也显示出对能量输出的调节作用。所提出的分子机制至少有四个保守特征：（i）存在膜屏障，（ii）质子驱动的ATP酶，（iii）ΔpH，以及（iv）腐胺池。
