CD58分子表达与乙型肝炎严重程度的相关性研究
Investigation on correlation between expression of CD58 molecule and severity of hepatitis B.
作者信息
Sheng Li, Li Jie, Qi Bao-Tai, Ji Yu-Qiang, Meng Zhao-Jun, Xie Ming
机构信息
Department of Pathogenic Microbiology and Immunology, Medical School of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
出版信息
World J Gastroenterol. 2006 Jul 14;12(26):4237-40. doi: 10.3748/wjg.v12.i26.4237.
AIM
To investigate the correlation between expression of CD58 and severity of hepatitis B.
METHODS
The level of soluble CD58 (sCD58) in serum of patients with hepatitis B was detected by enzyme-linked immunosorbent assay. The level of expression of membrane CD58 molecule in PBMC was detected by direct immunofluorescence. The levels of serumal TBIL, DBIL, IBIL, ALT and AST were detected by the automated biochemistry analyzer as well.
RESULTS
The levels of sCD58 in serum and membrane CD58 molecule in PBMC of patients with hepatitis B were significantly higher than that in normal controls (P < 0.05). Level of CD58 was related to the levels of serumal TBIL, DBIL, IBIL, ALT and AST.
CONCLUSION
The level of CD58 molecule (in both serum and PBMC form) of patients with hepatitis B is related to the degree of liver damage.
目的
探讨CD58表达与乙型肝炎严重程度之间的相关性。
方法
采用酶联免疫吸附测定法检测乙型肝炎患者血清中可溶性CD58（sCD58）水平。采用直接免疫荧光法检测外周血单个核细胞（PBMC）中膜CD58分子的表达水平。同时采用自动生化分析仪检测血清总胆红素（TBIL）、直接胆红素（DBIL）、间接胆红素（IBIL）、谷丙转氨酶（ALT）和谷草转氨酶（AST）水平。
结果
乙型肝炎患者血清中sCD58水平及PBMC中膜CD58分子水平显著高于正常对照组（P < 0.05）。CD58水平与血清TBIL、DBIL、IBIL、ALT和AST水平相关。
结论
乙型肝炎患者CD58分子水平（血清和PBMC形式）与肝损伤程度相关。
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