汞电极上硫醇末端标记DNA的自组装单分子层。
Self-assembled monolayers of thiol-end-labeled DNA at mercury electrodes.
作者信息
Ostatna Veronika, Palecek Emil
机构信息
Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, 612 65 Brno, Czech Republic.
出版信息
Langmuir. 2006 Jul 18;22(15):6481-4. doi: 10.1021/la061424v.
We show for the first time that thiol-end-labeled oligodeoxynucleotides form self-assembled monolayer (SAM) at Hg electrodes. Changes in voltammetric signals due to cytosine and Hg-S bond reduction indicated changes in positioning of the HS-(TTC)(7) molecules at the electrode with increasing HS-(TTC)7 concentration from lying flat with respect to SAMs, forming upright-standing molecules. This SAM behaved as an insulator not allowing electron transfer between [Ru(NH3)6]3+ and the electrode. Different adsorption modes of thiolated and thiol-free DNAs were observed.
我们首次证明，硫醇末端标记的寡脱氧核苷酸在汞电极上形成自组装单分子层（SAM）。由于胞嘧啶和汞 - 硫键还原导致的伏安信号变化表明，随着HS-(TTC)7浓度的增加，HS-(TTC)7分子在电极上的定位发生变化，从相对于SAM平躺状态转变为形成直立分子。这种SAM表现为绝缘体，不允许[Ru(NH3)6]3+与电极之间发生电子转移。观察到了硫醇化和无硫醇DNA的不同吸附模式。
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