通过用重组牙龈蛋白酶结构域免疫产生针对牙龈卟啉单胞菌菌株的保护性抗体。

Production of protective antibodies against Porphyromonas gingivalis strains by immunization with recombinant gingipain domains.

作者信息

Yasaki-Inagaki Yuriko, Inagaki Satoru, Yamada Satoru, Okuda Katsuji, Ishihara Kazuyuki

机构信息

Department of Periodontics, Tokyo Dental College, Chiba, Japan.

出版信息

FEMS Immunol Med Microbiol. 2006 Jul;47(2):287-95. doi: 10.1111/j.1574-695X.2006.00091.x.

DOI:10.1111/j.1574-695X.2006.00091.x

PMID:16831217

Abstract

We studied the effect of antibodies against Porphyromonas gingivalis gingipain domains, preparing them against three recombinant fragments of RgpA (catalytic domain, r-Rgp CAT; hemagglutinin domains, r-Rgp 44 and r-Rgps 15-27) and one fragment of Kgp (catalytic domain, r-Kgp CAT). Enhancement of opsonization and killing by human polymorphonuclear leukocytes were measured in the noninvasive FDC 381 and invasive W50 strains of P. gingivalis. Anti-r-Rgp 44 was the most effective in both strains of P. gingivalis. The present findings lead us to recommend RgpA 44 as a candidate immunogen for vaccines against P. gingivalis.

摘要

我们研究了抗牙龈卟啉单胞菌牙龈蛋白酶结构域抗体的作用，制备了针对RgpA的三个重组片段（催化结构域，r-Rgp CAT；血凝素结构域，r-Rgp 44和r-Rgps 15-27）以及Kgp的一个片段（催化结构域，r-Kgp CAT）的抗体。在牙龈卟啉单胞菌的非侵袭性FDC 381菌株和侵袭性W50菌株中，检测了人多形核白细胞调理吞噬作用和杀伤作用的增强情况。抗r-Rgp 44在牙龈卟啉单胞菌的两种菌株中最为有效。目前的研究结果使我们推荐RgpA 44作为抗牙龈卟啉单胞菌疫苗的候选免疫原。