Cai Jie, Gao Hui-juan, Huang He-feng
Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
Zhonghua Fu Chan Ke Za Zhi. 2006 Jun;41(6):372-4.
To investigate the role of follicle stimulating hormone receptor (FSHR) expression on granulosa cells in ovarian response of women undergoing gonadotropin stimulation.
A total of 60 women were recruited and divided into poor, moderate and high responders according to the number of follicles. FSHR expression was detemined by Western blot on granulosa cells obtained by follicular aspiration. The peak levels of serum estradiol (E(2)) were measured by immunofluorescent assay.
(1) The expression of FSHR was significantly different among the 3 groups, being 0.19 +/- 0.07, 0.34 +/- 0.16, and 0.45 +/- 0.18 for poor, moderate and high responders, respectively (P < 0.001). Poor responders had the lowest peak levels of serum E(2) and the least number of follicles. (2) The FSHR was positively correlated with peak level of E(2) in serum and the number of follicles (P < 0.01 for both).
Ovarian response to gonadotropin stimulation depends on the different levels of FSHR expression on granulosa cells.
探讨促卵泡激素受体（FSHR）在颗粒细胞上的表达在接受促性腺激素刺激的女性卵巢反应中的作用。
共招募60名女性，根据卵泡数量分为低反应者、中等反应者和高反应者。通过卵泡抽吸获得的颗粒细胞，采用蛋白质免疫印迹法检测FSHR表达。采用免疫荧光法测定血清雌二醇（E₂）的峰值水平。
（1）3组之间FSHR表达有显著差异，低反应者、中等反应者和高反应者的FSHR表达分别为0.19±0.07、0.34±0.16和0.45±0.18（P<0.001）。低反应者血清E₂峰值水平最低，卵泡数量最少。（2）FSHR与血清E₂峰值水平和卵泡数量呈正相关（两者P均<0.01）。
卵巢对促性腺激素刺激的反应取决于颗粒细胞上FSHR表达的不同水平。
Zotero 插件