负责杀伤靶细胞的小鼠淋巴因子激活的杀伤细胞上的黏附分子：CD2的作用。
Adhesion molecules on murine lymphokine-activated killer cells responsible for target cell killing: a role of CD2.
作者信息
Kato K, Tanabe T, Yagita H, Agatsuma T, Hojo H, Hashimoto Y
机构信息
Department of Hygienic Chemistry, Tohoku University, Sendai.
出版信息
Jpn J Cancer Res. 1991 Oct;82(10):1139-44. doi: 10.1111/j.1349-7006.1991.tb01768.x.
Lymphokine-activated killer (LAK) cells were induced from C57BL/6 mouse spleen cells and the effects of culture time on the expression of cell surface phenotypes and cytotoxic activity of LAK cells were determined. The expression of CD2 remarkably decreased after culture of LAK cells for 30 days, while LFA-1, a principal adhesion molecule in LAK cells, and CD3 were not changed by the culture. LAK cells cultured for 90 days completely lost CD2. In accordance with the decrease of CD2, the cytotoxic activity of LAK cells declined but a certain leven was retained even after the complete loss of CD2. The established LAK cell clones were also strongly positive for the expression of LFA-1 but negative for CD2. When the LAK cell clones were transfected with the CD2 cDNA, they started to express CD2 on their cell surface and to show greater binding ability and stronger cytotoxicity to target tumor cells. These results indicated that CD2 plays a role as an adhesion molecule responsible for target cell killing in murine LAK cells.
从C57BL/6小鼠脾细胞诱导出淋巴因子激活的杀伤（LAK）细胞，并测定培养时间对LAK细胞表面表型表达和细胞毒活性的影响。LAK细胞培养30天后，CD2的表达显著降低，而LAK细胞中的主要黏附分子LFA-1和CD3不受培养影响。培养90天的LAK细胞完全丧失CD2。随着CD2的减少，LAK细胞的细胞毒活性下降，但即使在CD2完全丧失后仍保留一定水平。所建立的LAK细胞克隆LFA-1表达也呈强阳性，但CD2呈阴性。当用CD2 cDNA转染LAK细胞克隆时，它们开始在细胞表面表达CD2，并对靶肿瘤细胞表现出更强的结合能力和更强的细胞毒性。这些结果表明，CD2在小鼠LAK细胞中作为负责杀伤靶细胞的黏附分子发挥作用。
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