Wang Sa, Huang Cui, Zheng Tie-li, Zhang Zhi-xing, Wang Yi-ning, Cheng Xiang-rong
Key Laboratory of Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2006 Jun;41(6):323-6.
To evaluate the microtensile bond strength and bond interface of total-etch or self-etch adhesives to normal dentin and caries-affected dentin.
A total of 20 molars with occlusal caries lesion were used. The caries-affected dentin was obtained by removing the caries-infected dentin under the guidance of the caries detector. Beyond the level of caries-affected dentin all the enamel and partial dentin were removed. The adhesive systems, two total-etch adhesives (All-Bond 2, Prime&Bond NT) and two self-etch adhesives (Clearfil SE Bond, Xeno III) were applied respectively under the instructions of manufacturers. A block of composite resin was build up superficially. All the teeth were sectioned to obtain bar-shaped specimens with bonded surface area about 0.9 mm x 0.9 mm. The specimens were divided into normal dentin group and caries-affected dentin group via stereomicroscope. The bond strength was tested in a microtensile tester with a crosshead speed of 1 mm/min. The mean values of bond strength were compared using two-way ANOVA. The bonding interface between the dentin and adhesives was qualitatively evaluated under the observation of scanning electron microscope (SEM).
Two-way ANOVA revealed a significant influence of both the type of dentin and the adhesive systems tested on microtensile bond strength values. All the adhesives attained higher strength in normal dentin. In normal dentin, there was no significant difference between total-etch and self-etch adhesives. In caries-affected dentin, bond strength of Xeno III was significantly lower than the others. For SEM, the hybrid layer in caries-affected dentin was thicker but more porous than that in normal dentin. Compared with normal dentin, there was fewer resin tag exhibited in caries-affected dentin and no lateral branches were observed.
The total-etch adhesive had higher bond strength than self-etch adhesive systems in caries-affected dentin.
评估全酸蚀或自酸蚀粘结剂与正常牙本质及龋损牙本质的微拉伸粘结强度和粘结界面。
使用20颗有咬合面龋损的磨牙。在龋病探测器的引导下，去除感染龋坏的牙本质以获取龋损牙本质。在龋损牙本质水平之外，去除所有釉质和部分牙本质。按照制造商的说明分别应用两种全酸蚀粘结剂（全粘结剂2、Prime&Bond NT）和两种自酸蚀粘结剂（Clearfil SE Bond、Xeno III）。在表面堆积一块复合树脂。将所有牙齿切片以获得粘结表面积约为0.9mm×0.9mm的条形标本。通过体视显微镜将标本分为正常牙本质组和龋损牙本质组。在微拉伸试验机上以1mm/min的十字头速度测试粘结强度。使用双向方差分析比较粘结强度的平均值。在扫描电子显微镜（SEM）观察下对牙本质与粘结剂之间的粘结界面进行定性评估。
双向方差分析显示，牙本质类型和所测试的粘结剂系统对微拉伸粘结强度值均有显著影响。所有粘结剂在正常牙本质中获得的强度更高。在正常牙本质中，全酸蚀粘结剂和自酸蚀粘结剂之间无显著差异。在龋损牙本质中，Xeno III的粘结强度显著低于其他粘结剂。对于SEM，龋损牙本质中的混合层比正常牙本质中的更厚但孔隙更多。与正常牙本质相比，龋损牙本质中显示的树脂突较少且未观察到侧支。
在龋损牙本质中，全酸蚀粘结剂的粘结强度高于自酸蚀粘结剂系统。
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