Fischer Karl P, Gares Sheryl L, Wang Dakun, Lorne Tyrrell D, Gutfreund Klaus S
Department of Medical Microbiology and Immunology, University of Alberta, 6-22 Heritage Medical Research Centre, Edmonton, Alberta, Canada T6G 2H6.
Dev Comp Immunol. 2007;31(1):61-71. doi: 10.1016/j.dci.2006.05.001. Epub 2006 Jun 6.
Binding of CD154, a member of the TNF ligand superfamily, to its receptor CD40 is essential for the development and regulation of adaptive immune responses in mammals. The duck CD154 (DuCD154) encoding gene was isolated from activated splenocytes using RT-PCR. Sequence analysis of the cloned DuCD154 gene revealed an open reading frame of 819 base pairs encoding a 272 amino acid protein. The extracellular domain of DuCD154 was identified and expressed for characterization and generation of antibodies. DuCD154 mRNA was predominantly expressed in spleen, thymus and duodenum. DuCD154 protein generated in cell culture was secreted and formed dimers. DuCD154 markedly enhanced proliferative responses in duck splenocytes when used alone or in conjunction with LPS or PHA. These observations suggest that DuCD154 has functional equivalence with mammalian CD154 and that the central role of CD154 as an immunoregulatory protein had already evolved before the divergence of birds and mammals.
肿瘤坏死因子配体超家族成员CD154与其受体CD40的结合对于哺乳动物适应性免疫反应的发展和调节至关重要。利用逆转录聚合酶链反应（RT-PCR）从活化的脾细胞中分离出鸭CD154（DuCD154）编码基因。对克隆的DuCD154基因进行序列分析，发现一个819个碱基对的开放阅读框，编码一个272个氨基酸的蛋白质。鉴定并表达了DuCD154的细胞外结构域，用于特性分析和抗体生成。DuCD154 mRNA主要在脾脏、胸腺和十二指肠中表达。在细胞培养中产生的DuCD154蛋白被分泌并形成二聚体。单独使用或与脂多糖（LPS）或植物血凝素（PHA）联合使用时，DuCD154显著增强鸭脾细胞的增殖反应。这些观察结果表明，DuCD154与哺乳动物CD154具有功能等效性，并且CD154作为免疫调节蛋白的核心作用在鸟类和哺乳动物分化之前就已经进化出来了。
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