体外胚胎干细胞中的甲基化。
Methylation in embryonic stem cells in vitro.
作者信息
Nishino Koichiro, Ohgane Jun, Suzuki Masako, Hattori Naka, Shiota Kunio
机构信息
Laboratory of Cellular Biochemistry, Animal Resource Sciences/Veterinary Medical Sciences, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo, Japan.
出版信息
Methods Mol Biol. 2006;329:421-45. doi: 10.1385/1-59745-037-5:421.
Stem cells raise the possibility of regenerating failing body parts with new tissue. Before stem cells can safely fulfill their promise, many technical problems, including understanding the stem cell phenotype, must be overcome. DNA methylation, which is responsible for gene silencing and is associated with chromatin remodeling, is an epigenetic system that determines the specific characteristic of a variety of cells, including stem cells. Each cell type has a unique DNA methylation profile produced by varied loci-specific methylation. Investigation of such DNA methylation profiles provides a way of identifying pluripotent stem cells. Further, it is likely that analysis of the epigenetic status of stem cells may provide novel information regarding "sternness" within these populations.
干细胞为利用新组织再生衰竭身体部位带来了可能性。在干细胞能够安全地实现其前景之前，必须克服许多技术问题，包括了解干细胞表型。DNA甲基化负责基因沉默并与染色质重塑相关，是一种表观遗传系统，它决定了包括干细胞在内的多种细胞的特定特征。每种细胞类型都有由不同的位点特异性甲基化产生的独特DNA甲基化图谱。对这种DNA甲基化图谱的研究提供了一种识别多能干细胞的方法。此外，对干细胞表观遗传状态的分析可能会提供有关这些细胞群体中“干性”的新信息。
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