[聚合酶链反应-单链构象多态性技术在法医线粒体DNA分型中的应用]
[The application of PCR-SSCP in forensic mtDNA typing].
作者信息
Ai Hong-Wei, Yu Chun-Ying, Zhao Gui-Sen, Huang Dai-Xin, Ke Ji, Yue Xia, Yang Qing-En
机构信息
Department of Forensic Medicine, Huazhong University of Science and Technology, Wuhan 430030, China.
出版信息
Fa Yi Xue Za Zhi. 2006 Apr;22(2):117-9.
OBJECTIVE
To study the application of PCR-SSCP in forensic mtDNA typing.
METHODS
Primers flanking the mtDNA HV-I and HV-II regions were designed. By PCR-SSCP techniques, 70 family trios and 140 unrelated Wuhan Han individuals were investigated and analyzed.
RESULTS
In 70 family trios, the SSCP profiles in region HV-I and HV-II of children were not same to that of their fathers in 98.57% and 97.13% respectively but were identical with their mothers. In 140 unrelated Wuhan Han individuals, 21 haplotypes were found in HVI, GD = 0.9556; 16 haplotypes were found in HVII, GD = 0.9356.
CONCLUSION
PCR-SSCP technique may be useful in forensic mtDNA typing, especially for screening the suspects.
目的
研究聚合酶链反应-单链构象多态性（PCR-SSCP）技术在法医线粒体DNA分型中的应用。
方法
设计侧翼线粒体DNA高变区I（HV-I）和高变区II（HV-II）的引物。采用PCR-SSCP技术对70个三联体家庭及140名武汉汉族无关个体进行调查分析。
结果
在70个三联体家庭中，子代HV-I区和HV-II区的SSCP图谱分别有98.57%和97.13%与父亲不同，而与母亲一致。在140名武汉汉族无关个体中，HV-I区发现21种单倍型，基因多样性（GD）=0.9556；HV-II区发现16种单倍型，GD = 0.9356。
结论
PCR-SSCP技术可用于法医线粒体DNA分型，尤其适用于嫌疑人的筛查。
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