Yu Zhi-gang, Zhen Jun-hui, Jia Hong-ying, Zhang Qiang, Sun Jing-zhong
Department of Breast Surgery, Second Affiliated of Shandong University, Jinan 250011, China.
Zhonghua Yi Xue Za Zhi. 2006 Jun 13;86(22):1558-63.
To investigate the relationships among the expression of thymidylate synthase (TS), thymidine phosphorylase (TP), and dihydropyrimidine dehydrogenase (DPD) and the prognosis of breast cancer.
Immunochemistry (ABC method) was used to detect the expression of TS, TP, and DPD in the samples of breast cancer resected during operation from 28 female patients. The microvessel density (MVD) in the cancer tissue was measured by immunochemistry (LSAB method).
The TS positive rate was 30.26% and the TP positive rate was 21%, and the DPD positive rate was 30.03%. The expression levels of TS and TP were both correlated with the tumor size, lymph node status, histological grading of tumor, and microvessel count (MVC) (all P < 0.01), and was not correlated with age, status of estrogen receptors, status of prasterone receptors (all P > 0.05). The DPD expression was not correlated with the age, tumor size, lymph node status, histological grading of tumor, status of estrogen receptors, status of prasterone receptors, and MVC. The ten-year disease-free survival rate of the TS-positive patients was 0, significantly lower than that of the TS-negative patients (25%, P < 0.01). The ten-year overall survival rate of the TS-positive patients was 3.9%, significantly lower than that of the TS-negative patients (58.8%, P < 0.01). The ten-year disease-free survival rate of the TP-positive patients was 0, significantly lower than the TP-negative patients (25.4%, P < 0.01). The ten-year overall survival rate of the TP-positive patients was 0.9%, significantly lower than that of the TP-negative patients (61.8%, P < 0.01). The ten-year disease-free survival rate and ten-year overall survival rate of the DPD positive patients were not significantly different from those of the DPD negative patients (both P > 0.05). MCV and TS expression were strong protective factors of disease-free survival rate and overall survival rate.
The levels of TS and TP are both prognostic indicis of breast cancer.
探讨胸苷酸合成酶（TS）、胸苷磷酸化酶（TP）和二氢嘧啶脱氢酶（DPD）的表达与乳腺癌预后的关系。
采用免疫化学法（ABC法）检测28例女性患者手术切除的乳腺癌标本中TS、TP和DPD的表达。采用免疫化学法（LSAB法）测定癌组织中的微血管密度（MVD）。
TS阳性率为30.26%，TP阳性率为21%，DPD阳性率为30.03%。TS和TP的表达水平均与肿瘤大小、淋巴结状态、肿瘤组织学分级及微血管计数（MVC）相关（均P<0.01），与年龄、雌激素受体状态、孕激素受体状态均无关（均P>0.05）。DPD表达与年龄、肿瘤大小、淋巴结状态、肿瘤组织学分级、雌激素受体状态、孕激素受体状态及MVC均无关。TS阳性患者的10年无病生存率为0，显著低于TS阴性患者（25%，P<0.01）。TS阳性患者的10年总生存率为3.9%，显著低于TS阴性患者（58.8%，P<0.01）。TP阳性患者的10年无病生存率为0，显著低于TP阴性患者（25.4%，P<0.01）。TP阳性患者的10年总生存率为0.9%，显著低于TP阴性患者（61.8%，P<0.01）。DPD阳性患者的10年无病生存率和10年总生存率与DPD阴性患者相比差异无统计学意义（均P>0.05）。MVC和TS表达是无病生存率和总生存率的强保护因素。
TS和TP水平均为乳腺癌的预后指标。
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