Watanaveeradej Veerachai, Sirirattanaphoomee Sudalak, Chantratita Wasun, Nitayaphan Sorachai, Viputtikul Kwanjai, Kerdpanich Angkool, Samakoses Rudiwilai, Simasathien Sriluk
Deparment of Pediatrics, Phramongkutklao Hospital, Bangkok, Thailand.
J Med Assoc Thai. 2005 Nov;88 Suppl 3:S206-13.
To develop a less expensive assay to calculate HIV-1 viral load for use in resource-limited countries.
An In-house One-tube-one-step Viral Load Assay (IOVA) was developed by using real-time PCR-based with TaqMan probe. Primers and probe were designed from the conserved region of sequences from all HIV subtypes. A standard curve was generated from reference virus in various dilutions. IOVA was applied on 105 HIV-positive and 25 HIV-negative samples and compared with the results from ROCHE AMPLICLOR.
IOVA measured HIV RNA in the samples ranging from 125 to 2 x 10(6) copies/mL. The coefficient of variation of intra- and inter-assay ranged from 0.68% to 7.89%. The sensitivity, specificity, positive and negative predictive values were 92%, 100%, 100% and 79.5% respectively. The parallel quantitative analysis showed high correlation (r=0.95) between IOVA and AMPLICOR.
A new HIV-1 viral load assay was developed and validated. It was reliable and less expensive.
开发一种成本较低的检测方法来计算HIV-1病毒载量，以供资源有限的国家使用。
采用基于TaqMan探针的实时PCR技术开发了一种内部单管一步病毒载量检测方法（IOVA）。引物和探针是根据所有HIV亚型序列的保守区域设计的。通过对不同稀释度的参考病毒生成标准曲线。将IOVA应用于105份HIV阳性样本和25份HIV阴性样本，并与罗氏AMPLICLOR的检测结果进行比较。
IOVA检测样本中的HIV RNA含量范围为125至2×10⁶拷贝/毫升。批内和批间变异系数范围为0.68%至7.89%。灵敏度、特异性、阳性预测值和阴性预测值分别为92%、100%、100%和79.5%。平行定量分析显示IOVA与AMPLICOR之间具有高度相关性（r = 0.95）。
开发并验证了一种新的HIV-1病毒载量检测方法。该方法可靠且成本较低。
