Tsuneishi Midori, Yamamoto Tatsuo, Kokeguchi Susumu, Tamaki Naofumi, Fukui Kazuhiro, Watanabe Tatsuo
Department of Oral Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8525, Japan.
Microbes Infect. 2006 Aug;8(9-10):2384-9. doi: 10.1016/j.micinf.2006.04.023. Epub 2006 Jun 30.
Tonsilloliths are a potential cause of oral malodor. In this study, microbial profiles and composition of tonsilloliths were determined using culture-independent molecular methods and scanning electron microscopy. 16S ribosomal RNA bacterial genes (16S rDNAs) isolated from tonsilloliths of 6 individuals were amplified by PCR and cloned into Escherichia coli. Partial 16S rDNA sequences of approximately 600 bases of cloned inserts were used to determine species identity by comparison with sequences of known species. Characteristics of bacteria on the surface and inside the tonsillolith were analyzed using scanning electron microscopy. Anaerobic bacteria detected in tonsilloliths belonged to the genera Eubacterium, Fusobacterium, Megasphaera, Porphyromonas, Prevotella, Selenomonas and Tannerella, all of which appear to be associated with production of volatile sulfur compounds. Electron microscopy revealed cocci and rods on the surface and rods predominating inside the tonsilloliths. These results support the tonsillolith as an origin of oral malodor.
扁桃体结石是口臭的一个潜在原因。在本研究中，使用非培养分子方法和扫描电子显微镜确定了扁桃体结石的微生物谱和组成。从6名个体的扁桃体结石中分离出的16S核糖体RNA细菌基因（16S rDNA）通过聚合酶链反应（PCR）进行扩增，并克隆到大肠杆菌中。通过与已知物种的序列比较，使用约600个碱基的克隆插入片段的部分16S rDNA序列来确定物种身份。使用扫描电子显微镜分析扁桃体结石表面和内部细菌的特征。在扁桃体结石中检测到的厌氧菌属于真杆菌属、梭杆菌属、巨球形菌属、卟啉单胞菌属、普雷沃菌属、硒单胞菌属和坦纳菌属，所有这些细菌似乎都与挥发性硫化合物的产生有关。电子显微镜显示扁桃体结石表面有球菌和杆菌，内部以杆菌为主。这些结果支持扁桃体结石是口臭的根源。
