Miyoshi Yukihiro, Okada Sanae, Uchimura Tai, Satoh Eiichi
Department of Applied Biology and Chemistry, Tokyo University of Agriculture, Setagaya, Tokyo 156-8502, Japan.
Biosci Biotechnol Biochem. 2006 Jul;70(7):1622-8. doi: 10.1271/bbb.50688.
Lactobacillus reuteri is one of the dominant lactobacilli found in the gastrointestinal tract of various animals. A surface protein of L. reuteri 104R, mucus adhesion promoting protein (MapA), is considered to be an adhesion factor of this strain. We investigated the relation between MapA and adhesion of L. reuteri to human intestinal (Caco-2) cells. Quantitative analysis of the adhesion of L. reuteri strains to Caco-2 cells showed that various L. reuteri strains bind not only to mucus but also to intestinal epithelial cells. In addition, purified MapA bound to Caco-2 cells, and this binding inhibited the adhesion of L. reuteri in a concentration-dependent manner. Based on these observations, the adhesion of L. reuteri appears due to the binding of MapA to receptor-like molecules on Caco-2 cells. Further, far-western analysis indicated the existence of multiple receptor-like molecules in Caco-2 cells.
罗伊氏乳杆菌是在各种动物胃肠道中发现的主要乳杆菌之一。罗伊氏乳杆菌104R的一种表面蛋白,即黏液黏附促进蛋白(MapA),被认为是该菌株的一种黏附因子。我们研究了MapA与罗伊氏乳杆菌对人肠道(Caco-2)细胞黏附之间的关系。对罗伊氏乳杆菌菌株与Caco-2细胞黏附的定量分析表明,各种罗伊氏乳杆菌菌株不仅与黏液结合,还与肠道上皮细胞结合。此外,纯化的MapA与Caco-2细胞结合,且这种结合以浓度依赖的方式抑制罗伊氏乳杆菌的黏附。基于这些观察结果,罗伊氏乳杆菌的黏附似乎是由于MapA与Caco-2细胞上的受体样分子结合所致。此外,Far-Western分析表明Caco-2细胞中存在多种受体样分子。