Koo Chuay-Yeng, Bay Boon-Huat, Lui Philip Chi-Wai, Tse Gary Man-Kit, Tan Puay-Hoon, Yip George Wai-Cheong
Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Mod Pathol. 2006 Oct;19(10):1344-50. doi: 10.1038/modpathol.3800657. Epub 2006 Jul 7.
Phyllodes tumors are fibroepithelial neoplasms typified by stromal proliferation. We have previously shown the role of pathologic parameters and the prognostic significance of p53 and CD117 protein expression in these tumors. In this study, we evaluated the expression of heparan sulfate, which has been implicated in many biological processes such as cell adhesion, embryogenesis, and tumorigenesis (including malignant transformation of mammary cells) in 232 breast phyllodes tumors. We used a monoclonal antibody, 10E4, to examine the localization of heparan sulfate in phyllodes tumors by immunohistochemistry. The immunoreactivity of both epithelial and stromal components was examined and analyzed with pathological parameters and other immunohistochemical markers, including p53, MIB1, bcl2, and CD117. Stromal 10E4 expression was significantly associated with tumor grade, stromal p53, and MIB1 expression in proliferating cells, suggesting that heparan sulfate may participate in malignant tumor growth.
叶状肿瘤是一种以间质增生为特征的纤维上皮性肿瘤。我们之前已经阐述了病理参数以及p53和CD117蛋白表达在这些肿瘤中的预后意义。在本研究中，我们评估了硫酸乙酰肝素的表达情况，硫酸乙酰肝素参与了许多生物学过程，如细胞黏附、胚胎发生和肿瘤发生（包括乳腺细胞的恶性转化）。我们对232例乳腺叶状肿瘤进行了研究。我们使用单克隆抗体10E4，通过免疫组织化学检测硫酸乙酰肝素在叶状肿瘤中的定位。同时检测并分析上皮和间质成分的免疫反应性，以及与病理参数和其他免疫组化标记物（包括p53、MIB1、bcl2和CD117）之间的关系。间质10E4表达与肿瘤分级、间质p53以及增殖细胞中的MIB1表达显著相关，提示硫酸乙酰肝素可能参与恶性肿瘤的生长。
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