Stewart Jelena, Ware Jeffrey, Fortina Paolo, Breaux Jim, Gulati Sandeep, Kennedy Ann
Department of Radiation Oncology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6072, USA.
Oncol Rep. 2006 Sep;16(3):569-74.
L-selenomethionine (SeM) is emerging as a highly effective protective agent against radiation-induced biological effects. We have shown its protective effect on space radiation-induced death of MCF-10 cells as well as on space radiation-induced transformation of HTori-3 cells. The present study was aimed at elucidation of molecular mechanisms and cellular pathways involved in SeM-mediated radioprotection. Human thyroid epithelial cells (HTori-3 cells), in the presence or absence of SeM, were exposed to a non-toxic or a slightly toxic radiation dose from 1 GeV/n iron ions (10 cGy and 20 cGy, respectively). Total RNA was prepared and changes in gene expression were analyzed using microarray technology. Our analysis has revealed a dramatic effect of SeM on alterations of gene expression caused by space radiation. This study provides a basis for furthering our knowledge about radiation-induced molecular and cellular changes that lead to cellular transformation and death.
L-硒代蛋氨酸（SeM）正成为一种针对辐射诱导生物效应的高效保护剂。我们已经证明了它对空间辐射诱导的MCF-10细胞死亡以及对空间辐射诱导的HTori-3细胞转化具有保护作用。本研究旨在阐明SeM介导的辐射防护所涉及的分子机制和细胞途径。人甲状腺上皮细胞（HTori-3细胞）在有或没有SeM的情况下，分别接受来自1 GeV/n铁离子的无毒或轻微毒性辐射剂量（分别为10 cGy和20 cGy）。制备总RNA，并使用微阵列技术分析基因表达的变化。我们的分析揭示了SeM对空间辐射引起的基因表达改变具有显著影响。这项研究为进一步了解导致细胞转化和死亡的辐射诱导分子和细胞变化奠定了基础。
