Ye Fanggui, Xie Zenghong, Wong Kwok-Yin
The MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, China.
Electrophoresis. 2006 Sep;27(17):3373-80. doi: 10.1002/elps.200500869.
A silica-based monolithic column as polar stationary phase is proposed for pressurized CEC (pCEC). The monolithic silica matrix from a sol-gel process was chemically modified by 3-aminopropyltrimethoxysilane to produce a column for hydrophilic interaction applications. The amino groups on the surface of the polar stationary phase generated anodic EOF under acidic conditions and served at the same time as a weak anion-exchanger. The anion solutes such as nucleotides were separated by the mixed mode mechanism, which comprised hydrophilic interaction, weak anion-exchange, and electrophoresis. The influences of buffer concentration and organic modifier content on the separation of nucleotides by pCEC have been investigated. In addition, the monolithic silica columns were also able to separate various polar compounds such as phenols, nucleic acid bases, and nucleosides in the hydrophilic interaction CEC mode.
提出了一种基于硅胶的整体柱作为极性固定相用于加压毛细管电色谱（pCEC）。通过溶胶 - 凝胶法制备的整体硅胶基质用3 - 氨丙基三甲氧基硅烷进行化学修饰，以制备用于亲水相互作用应用的色谱柱。极性固定相表面的氨基在酸性条件下产生阳极电渗流，同时作为弱阴离子交换剂。核苷酸等阴离子溶质通过混合模式机制进行分离，该机制包括亲水相互作用、弱阴离子交换和电泳。研究了缓冲液浓度和有机改性剂含量对pCEC分离核苷酸的影响。此外，整体硅胶柱在亲水相互作用CEC模式下也能够分离各种极性化合物，如酚类、核酸碱基和核苷。
