c-met小干扰RNA对肝癌MHCC97-H细胞生长及侵袭的影响
[Effects of c-met-siRNA on the growth and invasion of hepatocellular carcinoma MHCC97-H cells].
作者信息
Xie Bin, Tang De-guo, Dong Jia-hong
机构信息
Department of Hepatobiliary Surgery, Daping Hospital, Field Surgery Research Institute, Third Military Medical University, Chongqing 400042, China.
出版信息
Zhonghua Gan Zang Bing Za Zhi. 2006 Jul;14(7):499-504.
OBJECTIVE
To explore the effects of c-met-siRNA on the growth and invasion of hepatocellular carcinoma MHCC97-H cells by pSuppressorRetro/c-met-siRNA recombinant plasmid transfection.
METHODS
Recombinant plasmid transfection to Phoenix A cells was constructed using the lipofectin method and then the retrovirals containing c-met-siRNA were used to infect target cells MHCC97-H. In vitro, c-met expression was tested by Western blot. Cell proliferation, motility and invasiveness were studied using MTT, cell migration assay, and cell invasion assay, respectively.
RESULTS
The expression of c-met decreased significantly in MHCC97-H cells, and the most effective site of the target sequence was at 537. The growth, motility and invasiveness of MHCC97-H cells were inhibited.
CONCLUSION
The results indicate that c-met-siRNA can down-regulate the expression of c-met and inhibit hepatocellular carcinoma cell proliferation, motility and invasiveness.
目的
通过pSuppressorRetro/c-met-siRNA重组质粒转染，探讨c-met-siRNA对肝癌MHCC97-H细胞生长和侵袭的影响。
方法
采用脂质体法构建重组质粒转染Phoenix A细胞，然后用含c-met-siRNA的逆转录病毒感染靶细胞MHCC97-H。体外通过蛋白质免疫印迹法检测c-met表达。分别采用MTT法、细胞迁移实验和细胞侵袭实验研究细胞增殖、运动能力和侵袭能力。
结果
MHCC97-H细胞中c-met表达显著降低，靶序列最有效的位点在537处。MHCC97-H细胞的生长、运动能力和侵袭能力受到抑制。
结论
结果表明，c-met-siRNA可下调c-met表达，抑制肝癌细胞增殖、运动能力和侵袭能力。
Zotero 插件