Vila J, Esplugues J V, Martinez-Cuesta M A, Martinez-Martinez M C, Aldasoro M, Flor B, Lluch S
Department of Physiology, University of Valencia, Spain.
J Pharm Pharmacol. 1991 Dec;43(12):869-70. doi: 10.1111/j.2042-7158.1991.tb03198.x.
The L-arginine analogues NG-monomethyl-L-arginine (L-NMMA, 10(-4) M) and NG-nitro-L-arginine methyl ester (L-NAME, 10(-4) M), which specifically inhibit the synthesis of nitric oxide from L-arginine, significantly reduced acetylcholine-induced endothelium-dependent relaxations in rings of human omental arteries. The inhibitory potency of L-NMMA and L-NAME was similar. Addition of L-NMMA or L-NAME to the organ bath did not induce any significant changes in the resting tension of the tissues. The effects of L-NMMA were reversed by L-arginine (3 x 10(-4) M). The L-NMMA enantiomer, D-NMMA (10(-4) M), did not influence either the basal tone of the preparation or the relaxing effects of acetylcholine. Arterial relaxations induced by sodium nitroprusside (10(-6) M) were not influenced by incubation with L-NMMA or L-NAME. These results suggest that endothelium-dependent relaxations in human omental arteries are mediated by the endogenous and substrate-specific generation of nitric oxide from L-arginine.
L-精氨酸类似物NG-单甲基-L-精氨酸（L-NMMA，10⁻⁴ M）和NG-硝基-L-精氨酸甲酯（L-NAME，10⁻⁴ M）可特异性抑制由L-精氨酸合成一氧化氮，它们显著降低了乙酰胆碱诱导的人网膜动脉环内皮依赖性舒张。L-NMMA和L-NAME的抑制效力相似。向器官浴中添加L-NMMA或L-NAME不会引起组织静息张力的任何显著变化。L-精氨酸（3×10⁻⁴ M）可逆转L-NMMA的作用。L-NMMA的对映体D-NMMA（10⁻⁴ M）既不影响标本的基础张力，也不影响乙酰胆碱的舒张作用。硝普钠（10⁻⁶ M）诱导的动脉舒张不受与L-NMMA或L-NAME孵育的影响。这些结果表明，人网膜动脉的内皮依赖性舒张是由L-精氨酸内源性和底物特异性生成一氧化氮介导的。
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