Papanastasiou Anastasios D, Georgaka Eleftheria, Zarkadis Ioannis K
Department of Biology, School of Medicine, University of Patras, Rion, 26500 Patras, Greece.
Mol Immunol. 2007 Feb;44(6):1300-6. doi: 10.1016/j.molimm.2006.05.014. Epub 2006 Jul 28.
CD59, the major inhibitor of the complement membrane attack complex, is an 18-20 kDa glycoprotein, linked to the membrane via a glycosylphosphatidylinositol (GPI)-anchor. It restricts binding of C9 to the C5b-8 complex, preventing the formation of the complement membrane attack complex C5b-9. In this study we report the cloning of a second CD59-like gene in the rainbow trout, Oncorhynchus mykiss (referred to as CD59-2 and the previously deposited trout CD59-like gene as CD59-1). Trout CD59-2 is 56% identical to CD59-1 at the amino acid level. Both of trout CD59s show the highest identity score (54%) with putative CD59-like molecules from other teleost, and the overall identity with their mammalian orthologs is less than 30%. Trout CD59s are expressed in brain, heart, intestine, kidney, liver and spleen. Particularly, CD59-2 is abundant in trout brain, while CD59-1 seems to be absent in the trout spleen. Moreover, both of trout CD59 genes seems to be present as a single copy in trout genome.
补体膜攻击复合物的主要抑制剂CD59是一种18 - 20 kDa的糖蛋白，通过糖基磷脂酰肌醇（GPI）锚定连接到膜上。它限制C9与C5b - 8复合物的结合，从而阻止补体膜攻击复合物C5b - 9的形成。在本研究中，我们报告了虹鳟（Oncorhynchus mykiss）中第二个CD59样基因的克隆（称为CD59 - 2，之前已存入的虹鳟CD59样基因称为CD59 - 1）。虹鳟CD59 - 2在氨基酸水平上与CD59 - 1有56%的同一性。虹鳟的两种CD59与其他硬骨鱼的推定CD59样分子显示出最高的同一性得分（54%），并且与它们的哺乳动物直系同源物的总体同一性小于30%。虹鳟CD59在脑、心脏、肠道、肾脏、肝脏和脾脏中表达。特别地，CD59 - 2在虹鳟脑中含量丰富，而CD59 - 1在虹鳟脾脏中似乎不存在。此外，虹鳟的两种CD59基因在虹鳟基因组中似乎都以单拷贝形式存在。
Zotero 插件