Wen Yue, Huang Xiang-feng, Qiu Zhan, Wang Feng, Zhang Fei-juan, Zhou Qi
State Key Laboratory of Pollution Control and Resources Reuses, Tongji University, Shanghai 200092, China.
Huan Jing Ke Xue. 2006 Jul;27(7):1362-8.
Hydrolysis-acidification + aerobic biological processes were conducted experimentally to treat oilfield wastewater pretreated with physical and chemical treatment in Xinjiang oilfield. The results showed that when the COD concentration in influent was 190-220 mg x L(-1), that in effluent reduced to 65-75 mg x L(-1) under HRT of 10h in both hydrolysis-acidification process and aerobic biological process, reaching the strictest requirement of Effluent Standards for Wastewater from Petroleum Development Industry (GB3550-83). Using GC/MS technology, the relative content of various organic pollutants was analyzed to discover the transfer and degradation law in the oilfield wastewater in biological treatment process. The system of DNA extraction technique, PCR and DGGE reacting systems were practical to analyze the microbial community in the hydrolysis-acidification and aerobic biological processes. The predominant sequences of several 16S rDNA DGGE fragments were determined and confirmed in comparison in GeneBank (NCBI).
采用水解酸化+好氧生物工艺对新疆油田经物理化学预处理的油田废水进行了实验处理。结果表明,当进水COD浓度为190220mg·L-1时,水解酸化和好氧生物工艺在HRT为10h的条件下,出水COD浓度降至6575mg·L-1,达到《石油开发工业废水排放标准》(GB3550-83)最严格要求。利用GC/MS技术分析了各种有机污染物的相对含量,以揭示生物处理过程中油田废水中有机污染物的迁移和降解规律。DNA提取技术、PCR和DGGE反应体系可用于分析水解酸化和好氧生物工艺中的微生物群落。通过与基因库(NCBI)比对,确定并证实了几个16S rDNA DGGE片段的优势序列。