[猪角质形成细胞在猪小肠黏膜下层体外培养的实验]
[The experiment of porcine keratinocytes cultured on porcine small intestinal submucosa in vitro].
作者信息
Fan Wei-jie, Yang Zhi-ming, Li Xiu-qun, Wang Zhen, Zhi Wei, Qiu Lin
机构信息
Division of Stem Cell and Tissue Engineering State Key Laboratory of Biotherapy, West China Hospital, Sichuan University.
出版信息
Zhonghua Zheng Xing Wai Ke Za Zhi. 2006 May;22(3):220-2.
OBJECTIVE
To investigate the feasibility of acellular porcine small intestinal submucosa (SIS as bioscaffold of tissue engineering skin.
METHODS
The second passage keratinocytes were seeded on SIS, after seeded for 11, 13, 15, 17 days, the keratinocytes/SIS composites were observed by dye directly, histopathology, immunohistochemical studies with monoclonal antibodies against laminin and transmission electron microscope (TEM).
RESULTS
At eleventh day, keratinocytes were growth very well on the surface of SIS, there are 2-3 cell layers on partial of the SIS surface, the continued expression of laminin can be detected between the keratinocytes and the surface of SIS. After 13, 15, 17 days this stratified structure increased, cells contact more closely, the tonofibrils in cells, desmosome between cells and the basal membrane between the keratinocytes and the surface of SIS can be seen with TEM.
CONCLUSIONS
SIS is a kind of good bioscaffold in the culture of porcine keratinocytes in vitro.
目的
探讨脱细胞猪小肠黏膜下层（SIS）作为组织工程皮肤生物支架的可行性。
方法
将第二代角质形成细胞接种于SIS上，接种11、13、15、17天后，通过直接染色、组织病理学、抗层粘连蛋白单克隆抗体免疫组织化学研究及透射电子显微镜（TEM）观察角质形成细胞/SIS复合物。
结果
第11天时，角质形成细胞在SIS表面生长良好，部分SIS表面有2 - 3层细胞，可检测到角质形成细胞与SIS表面之间层粘连蛋白的持续表达。13、15、17天后，这种分层结构增加，细胞接触更紧密，通过TEM可看到细胞内的张力原纤维、细胞间的桥粒以及角质形成细胞与SIS表面之间的基底膜。
结论
SIS是体外培养猪角质形成细胞的一种良好生物支架。
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