将TetR诱导肽标签随机插入大肠杆菌蛋白中，可通过诱导报告基因表达来分析蛋白水平。
Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression.
作者信息
Schlicht Maximilian, Berens Christian, Daam Janko, Hillen Wolfgang
机构信息
Lehrstuhl für Mikrobiologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Staudtstrasse 5, D-91058 Erlangen, Germany.
出版信息
Appl Environ Microbiol. 2006 Aug;72(8):5637-42. doi: 10.1128/AEM.00739-06.
Abstract
The insertion element InsTipalpha was constructed to generate protein expression data. It randomly fuses the TetR-inducing peptide Tip to the affected reading frame. Fusion protein expression is quantified by Tet-regulated reporter gene expression. The expression patterns of tagged Escherichia coli genes fully agree with published data from transcriptional fusions or microarrays, validating the Tip tag approach.
摘要
插入元件InsTipalpha被构建用于生成蛋白质表达数据。它将TetR诱导肽Tip随机融合到受影响的阅读框中。融合蛋白的表达通过Tet调控的报告基因表达进行定量。标记的大肠杆菌基因的表达模式与转录融合或微阵列的已发表数据完全一致，验证了Tip标签方法。
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